Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 414 Official Publication of Indian Association of Medical Microbiologists 
  Search
 
  
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~  Article in PDF (1,118 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 
 ~  References
 ~  Article Tables

 Article Access Statistics
    Viewed1971    
    Printed28    
    Emailed0    
    PDF Downloaded261    
    Comments [Add]    

Recommend this journal

 


 
  Table of Contents  
CORRESPONDENCE
Year : 2016  |  Volume : 34  |  Issue : 1  |  Page : 123-124
 

Evaluation of the antibiotic resistance of Pseudomonas aeruginosa isolates according to the changing guidelines


1 Department of Medical Microbiology, Faculty of Medical, Ondokuz Mayis University, Samsun, Turkey
2 Department of Infectious Diseases, Faculty of Medical , Ondokuz Mayis University, Samsun, Turkey
3 Department of Medical Microbiology, Faculty of Medical, Istanbul University, Istanbul, Turkey

Date of Submission30-Oct-2014
Date of Acceptance06-May-2015
Date of Web Publication15-Jan-2016

Correspondence Address:
Y Tanriverdi Cayci
Department of Medical Microbiology, Faculty of Medical, Ondokuz Mayis University, Samsun
Turkey
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.174107

Rights and Permissions



How to cite this article:
Yanik K, Cayci Y T, Karadag A, Esen S, Gunaydin M. Evaluation of the antibiotic resistance of Pseudomonas aeruginosa isolates according to the changing guidelines. Indian J Med Microbiol 2016;34:123-4

How to cite this URL:
Yanik K, Cayci Y T, Karadag A, Esen S, Gunaydin M. Evaluation of the antibiotic resistance of Pseudomonas aeruginosa isolates according to the changing guidelines. Indian J Med Microbiol [serial online] 2016 [cited 2020 Apr 8];34:123-4. Available from: http://www.ijmm.org/text.asp?2016/34/1/123/174107


Sir,

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that commonly found in nature.[1] Resistance to antibiotics and disinfectants is a problem in healthcare settings [2] some regulations are made in susceptibility testing and interpretation of results. Clinical and Laboratory Standards Institute (CLSI) guideline changed the minimum inhibitory concentrations (MICs) of piperacillin, piperacillin tazobactam, imipenem and meropenem which are used in the treatment of Pseudomonas infections, in 2012.[3]

The aim of this study was; evaluating MIC values of P. aeruginosa isolates which were isolated from clinical specimens according to the CLSI 2011.[4] CLSI 2012[3] and European Committee on Antimicrobial Susceptibility Testing (EUCAST)[5] guidelines, to reveal differences in susceptibility rates and to contribute to the debate on guide exchange.

A total of 3632 strains for imipenem, 3706 strains for meropenem, 3479 strains for piperacillin and 4760 strains for piperacillin-tazobactam were enrolled in this study. Identification of the strains and MIC values have been studied in Phoenix (BD Diagnostic, ABD) and Vitek2 (Biomerieux, France) automated systems.

When compared CLSI 2011 and 2012 criteria, it was observed that the rate of imipenem-susceptible strains were decreased from 58% to 54 5% and the rate of intermediate strains were increased from 4% to 7 5%. Furthermore, similar changes have been seen for meropenem and 4% decrease was observed for susceptible strains. However, 10% decrease was determined for the rate of susceptible strains for piperacillin and piperacillin-tazobactam [Table 1]. According to EUCAST criteria, while MIC values for imipenem is similar to CLSI 2011, susceptible strain rate same with CLSI 2011 and it was determined to be higher according to CLSI 2012. MIC values in EUCAST for piperacillin and piperacillin-tazobactam is lower and the susceptibility MIC value is same with CLSI 2012.
Table 1: Comparing of antibiotics which changes have been made in Pseudomonas aeruginosa MIC values and MIC results

Click here to view


 
 ~ References Top

1.
Erdem B. Pseudomonaslar. In: Mutlu G, Emir T, Cengiz AT, Ustacelebi S, Tumbay E, Mete O, editors. Basic and Clinical Microbiology, Ankara, Gunes Publishing; 1999. p. 733-8.  Back to cited text no. 1
    
2.
Lambert PA. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J R SocMed 2002;95 Suppl 41:22-6.  Back to cited text no. 2
    
3.
Clinical Laboratory and Standards Institute. Performance standards for antimicrobial susceptibility testing: Twenty-second international supplement, M100-S22. Wayne, PA: CLSI; 2012.  Back to cited text no. 3
    
4.
Clinical Laboratory and Standards Institute. Performance standards for antimicrobial susceptibility testing: Twenty-second international supplement, M100-S21. Wayne, PA: CLSI; 2011.  Back to cited text no. 4
    
5.
Available from: http://www.eucast.org/clinical_breakpoints.  Back to cited text no. 5
    



 
 
    Tables

  [Table 1]



 

Top
Print this article  Email this article
 

    

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04