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Year : 2015  |  Volume : 33  |  Issue : 5  |  Page : 53-58

A method for early detection of antibiotic resistance in positive blood cultures: Experience from an oncology centre in eastern India

1 Department of Clinical Microbiology, Tata Medical Center, Kolkata, West Bengal, India
2 Laboratory Technologist in Microbiology, Tata Medical Center, Kolkata, West Bengal, India
3 Consultant Microbiologist, Tata Medical Center, Kolkata, West Bengal, India

Correspondence Address:
S Bhattacharya
Consultant Microbiologist, Tata Medical Center, Kolkata, West Bengal
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.150883

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Purpose: For antibiotic susceptibility results, conventional culture and sensitivity methods takes 48 hours after a blood culture is flagged positive by automated systems. Early initiation of targeted antibiotic therapy is essential for effective management of sepsis to reduce morbidity, mortality, cost of treatment and prevent antibiotic resistance. Objective of this study was to evaluate Direct Sensitivity Test (DST) as a potential tool to get reliable antibiotic susceptibility results 24 hours earlier. Materials and Methods: Blood cultures flagged positive between May 2011 to December 2012 by BacT/ALERT were Gram stained. All uni-microbial gram-negative blood cultures were simultaneously cultured and processed for DST from broth using disk diffusion method using British Society of Antimicrobial Chemotherapy (BSAC) guidelines. DST results available next day were compared with conventional antibiotic susceptibility test (AST) performed by Vitek-2 on isolated colonies. Results of DST (test method) and AST (reference method) were compared for agreements or errors. Results: Of the 840 antibiotic gram-negative organism combinations tested, Categorical and essential agreements were 83.7% and 96.2% respectively. Minor, major and very major errors were 12.5%, 3.33% and 0.47%, respectively. Conclusions: DST using disk diffusion from positive blood culture broths helps to initiate early targeted antibiotic therapy. There is high concordance between DST and AST.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04