Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 5965 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed3505    
    Printed89    
    Emailed1    
    PDF Downloaded154    
    Comments [Add]    

Recommend this journal

 

 BRIEF COMMUNICATION
Year : 2015  |  Volume : 33  |  Issue : 4  |  Page : 568-571

Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis


1 Ocular Microbiology Service, L V Prasad Eye Institute, Bhubaneswar, Odisha, India
2 Retina-Vitreous and Uveitis Services, L V Prasad Eye Institute, Bhubaneswar, Odisha, India
3 Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India

Correspondence Address:
S Sharma
Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana
India
Login to access the Email id

Source of Support: Department of Science and Technology, Government of India, New Delhi, India, Grant no. SR/FT/LS-15/2011, awarded to Soumyava Basu and Hyderabad Eye Research Foundation, Hyderabad. Praveen Kumar Balne is supported by a senior research fellowship from the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, India, Conflict of Interest: None


DOI: 10.4103/0255-0857.167339

Rights and Permissions

This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04