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Year : 2015  |  Volume : 33  |  Issue : 4  |  Page : 482-490

How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India

Departments of Microbiology and Clinical Hematology, Tata Medical Center, Rajarhat, Kolkata, India

Correspondence Address:
Sanjay Bhattacharya
Departments of Microbiology and Clinical Hematology, Tata Medical Center, Rajarhat, Kolkata
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.167351

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Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04