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Year : 2015  |  Volume : 33  |  Issue : 2  |  Page : 293-295

A cost-effective carbohydrate fermentation test for yeast using microtitre plate


Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Pondicherry, India

Correspondence Address:
A Kali
Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Pondicherry
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.154884

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Carbohydrate fermentation test is a well-established technique for speciation of bacteria and fungi. However, long incubation period, cost and procedural complexity may limit its use. Here, we describe a simple modification of conventional carbohydrate fermentation technique using microtitre plate. Thirty-one yeast isolates were identified based on their fermentation property by microtitre plate method and results were compared with conventional method. The modified method had 85.7% sensitivity and 100% specificity. The average time taken to produce positive reaction was 24 hours. When compared with conventional method, modified method reduced turn-around time and cost of processing without significant increase in discordant results.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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