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  Table of Contents  
CORRESPONDENCE
Year : 2015  |  Volume : 33  |  Issue : 1  |  Page : 187
 

Detection of various types of resistance patterns and their correlation with minimum inhibitory concentrations against clindamycin among methicillin resistant S. aureus isolates


1 Department of Microbiology, Guru Gobind Singh Medical College and Hospital, Faridkot, India
2 Department of Microbiology, Chintpurni Medical College and Hospital, Pathankot, Punjab, India

Date of Submission04-Sep-2013
Date of Acceptance13-Mar-2014
Date of Web Publication5-Jan-2015

Correspondence Address:
N Jindal
Department of Microbiology, Guru Gobind Singh Medical College and Hospital, Faridkot
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.148437

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How to cite this article:
Arora S, Jindal N, Sharma V. Detection of various types of resistance patterns and their correlation with minimum inhibitory concentrations against clindamycin among methicillin resistant S. aureus isolates. Indian J Med Microbiol 2015;33:187

How to cite this URL:
Arora S, Jindal N, Sharma V. Detection of various types of resistance patterns and their correlation with minimum inhibitory concentrations against clindamycin among methicillin resistant S. aureus isolates. Indian J Med Microbiol [serial online] 2015 [cited 2019 Sep 18];33:187. Available from: http://www.ijmm.org/text.asp?2015/33/1/187/148437


Dear Editor,

We read an article "Detection of various types of resistance patterns and their correlation with minimum inhibitory concentrations against clindamycin among methicillin-resistant Staphylococcus aureus isolates" published in Indian Journal of Medical Microbiology 2012;30:165-9. [1] The authors have targeted a very critical issue of identification of inducible MLS B resistance among MRSA isolates as therapeutic failure is common with inducible type of clindamycin resistance. However, a basic clarification is needed.

For detecting MRSA isolates, the authors have used Kirby Bauer's Disc Diffusion method with 1-μg oxacillin disc which requires supplementation of Mueller Hinton Agar with 2% NaCl and incubation at 35°C. [1] In Clinical and Laboratory Standards Institute (CLSI) guidelines 2006 (quoted by the authors), cefoxitin disc (30 μg) has been recommended for detection of MRSA. [2] Other studies including the one conducted by us also indicated that cefoxitin disc is far superior to other phenotypic methods and its results are in concordance with the PCR for mecA gene for MRSA detection. [3],[4] CLSI guidelines (2011) state that cefoxitin is the surrogate marker for detection of MRSA. [5] Although use of oxacillin disc might not have varied the overall results of the study but as far as detection of MRSA is considered, cefoxitin disc would have been the better alternative as it is the potent inducer of mecA regulatory system, easy to put up and interpret.

In clinical microbiology laboratory, accurate and early detection of multidrug-resistant (MDR) strains is the key for the better prognosis of infections and prevention of spread of antibiotic resistance. Small modifications in routine laboratory practices guided by standard references such as CLSI might help a lot to achieve this goal.

 
 ~ References Top

1.
Sireesha P, Setty CR. Detection of various types of resistance patterns and their correlation with minimal inhibitory concentrations against clindamycin among methicillin-resistant Staphylococcus aureus isolates. Indian J Med Microbiol 2012;30:165-9.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
2.
Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement M100-S16. Wayne: Clinical and Laboratory Standards Institute; 2006.  Back to cited text no. 2
    
3.
Anand KB, Agrawal P, Kumar S, Kapila K. Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian J Med Microbiol 2009;27:27-9.  Back to cited text no. 3
[PUBMED]  Medknow Journal  
4.
Arora S, Devi P, Arora U, Devi B. Prevalence of Methicillin-resistant Staphylococcus Aureus (MRSA) in a Tertiary Care Hospital in Northern India. J Lab Physicians 2010;2:78-81.  Back to cited text no. 4
[PUBMED]  Medknow Journal  
5.
Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; twenty first informational supplement M100-S21. Wayne: Clinical and Laboratory Standards Institute;  2011.  Back to cited text no. 5
    




 

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