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  Table of Contents  
COMMENTARY
Year : 2015  |  Volume : 33  |  Issue : 1  |  Page : 147
 

GenoType  MTBDR assay for detection of rifampicin and isoniazid resistance


Visiting Professor, Hainan Medical University, China; Visiting Professor, Faculty of Medicine, University of Nis, Serbia; Adjunct Professor, Joseph Ayobabalola University, Nigeria

Date of Submission10-Jan-2014
Date of Acceptance10-Feb-2014
Date of Web Publication5-Jan-2015

Correspondence Address:
Viroj Wiwanitkit
Visiting Professor, Hainan Medical University, China; Visiting Professor, Faculty of Medicine, University of Nis, Serbia; Adjunct Professor, Joseph Ayobabalola University, Nigeria

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Source of Support: None, Conflict of Interest: None


PMID: 25560021

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How to cite this article:
Wiwanitkit V. GenoType  MTBDR assay for detection of rifampicin and isoniazid resistance. Indian J Med Microbiol 2015;33:147

How to cite this URL:
Wiwanitkit V. GenoType  MTBDR assay for detection of rifampicin and isoniazid resistance. Indian J Med Microbiol [serial online] 2015 [cited 2019 Dec 6];33:147. Available from: http://www.ijmm.org/text.asp?2015/33/1/147/148421


Dear Editor,

Tuberculosis is a worldwide public health problem. Millions of world populations get this infection that leads to morbidity and mortality. Diagnosis of tuberculosis is the key point for successful management of the disease. Early diagnosis is the aim in diagnostic medical microbiology. Classically, to diagnose tuberculosis is based on the microscopic examination of the acid-fast stain of smear specimen. Although this technique is cheap and widely used, it has many limitations. First, the low sensitivity of the test can be expected. False negative result is common. In addition, the positive result can tell the physician that there is an infection by tuberculous pathogen; however, it cannot tell the exact natures of the pathogen including to the resistance to antituberculotic drug. With worldwide spreading of HIV infection, the complicated tuberculosis can be easily seen and becomes the problem in laboratory diagnosis. The report on the "GenoType MTBDR Assay for Detection of Rifampicin and Isoniazid Resistance" is an interesting article. [1]

In fact, the drug resistance is an important problem in tuberculosis management. To manage the problem, the diagnosis of resistance is the first step. With advent in molecular biology, the use of the PCR tool for determination of mutation contributing to drug resistance can be possible. GenoType MTBDR Assay is a good example of new tool. It has been evaluated and confirmed for its advantage in several settings such as Turkey and Germany. [2],[3] As a rule in laboratory medicine, the evaluation of the clinical usefulness of the new diagnostic test is needed before implementation in any setting. The present report can confirm the usefulness of GenoType MTBDR Assay. [1] Nevertheless, there are also some points to be mentioned. First, the unit cost of GenoType MTBDR Assay might be expensive and this limits the implementation of the test to the resource-limited settings where the problems of drug resistance tuberculosis are usually prevalent. Also, the recent report in 2014 by Lyu et al. from Korea showed that "the sensitivity in detecting INH resistance and MDR-TB was not optimal (< 95%)". [4] Hence, the problem in using GenoType MTBDR Assay still exists.

If we would like to say that GenoType MTBDR is a modern technique, it should also be noted that there are also other more advent options for diagnosis of drug-resistant tuberculosis. The oligonucleotide array chip with automatic reader is the good example that can give the comparable diagnostic activity comparing to GenoType MTBDR. [5],[6]

 
 ~ References Top

1.
Saglik I, Oz Y, Kiraz N. Evaluation of the GenoType MTBDR assay for detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex isolates. Indian J Med Microbiol 2014;32:318-22.  Back to cited text no. 1
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2.
Aslan G, Tezcan S, Emekdaº G. Evaluation of the genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in clinical Mycobacterium tuberculosis complex clinical isolates. Mikrobiyol Bul 2009;43:217-6.  Back to cited text no. 2
    
3.
Hillemann D, Rüsch-Gerdes S, Richter E. Evaluation of the GenoType MTBDR plus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens. J Clin Microbiol 2007;45:2635-40.  Back to cited text no. 3
    
4.
Lyu J, Kim MN, Song JW, Choi CM, Oh YM, Lee SD, et al. GenoType® MTBDRplus assay detection of drug-resistant tuberculosis in routine practice in Korea. Int J Tuberc Lung Dis 2013;17:120-4.  Back to cited text no. 4
    
5.
Huang WL, Hsu ZJ, Chang TC, Jou R. Rapid and accurate detection of rifampin and isoniazid-resistant Mycobacterium tuberculosis using an oligonucleotide array. Clin Microbiol Infect 2013.  Back to cited text no. 5
    
6.
Park H, Song EJ, Song ES, Lee EY, Kim CM, Jeong SH, et al. Comparison of a conventional antimicrobial susceptibility assay to an oligonucleotide chip system for detection of drug resistance in Mycobacterium tuberculosis isolates. J Clin Microbiol 2006;44:1619-24.  Back to cited text no. 6
    




 

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