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CORRESPONDENCE
Year : 2014  |  Volume : 32  |  Issue : 4  |  Page : 467-468
 

Biofilm formation by Candida species on intrauretheral catheter and its antifungal susceptibility profile


Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, Delhi-110 095, India

Date of Submission16-Sep-2013
Date of Acceptance29-Jan-2014
Date of Web Publication4-Oct-2014

Correspondence Address:
S Das
Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, Delhi-110 095
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.142244

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How to cite this article:
Chaudhary S, Gupta C, Das S, Saha R, Rani M, Ramachandran V G. Biofilm formation by Candida species on intrauretheral catheter and its antifungal susceptibility profile . Indian J Med Microbiol 2014;32:467-8

How to cite this URL:
Chaudhary S, Gupta C, Das S, Saha R, Rani M, Ramachandran V G. Biofilm formation by Candida species on intrauretheral catheter and its antifungal susceptibility profile . Indian J Med Microbiol [serial online] 2014 [cited 2019 Nov 13];32:467-8. Available from: http://www.ijmm.org/text.asp?2014/32/4/467/142244


Dear Editor,

Indwelling urinary catheter is the single most predisposing factor implicated in aetiopathogenesis of hospital-acquired urinary tract infections (UTI). [1] High incidence of catheter-associated UTI (CAUTI) is due to biofilm mode of growth of microbes. In light of inadequacy of literature on the fungal biofilms, this study was initiated to study antimicrobial-resistance pattern in biofilm-forming fungal isolates in CAUTI.

Over the period of 1 year, 100 Candida species were speciated from catheterised urine samples and their biofilm formation was determined by spectrophotometric method. [2] Only biofilm forming isolates were further tested to determine antifungal-resistance pattern. Antifungal susceptibility profile of planktonic cells of biofilm-forming isolates was done by determining Minimum Inhibitory Concentration (MIC) by broth microdilution method. [3] In vitro antifungal susceptibility profile of biofilm-adherent cells was done by exposing biofilms to the antifungals and determining MIC. [4] Candida albicans ATCC 90028 and Candida parapsilosis ATCC 96142 were used as controls. Biofilm formation was seen in 54 percent of Candida species isolates. MIC determination showed that planktonic cells were susceptible to antifungals. Only 72.2% biofilm adherent cells were sensitive to amphotericin B while the sensitivity for azoles was negligible [Table 1].
Table 1: Antifungal susceptibility of planktonic and biofilm-adherent cells of Candida species


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The artificial surface of catheter facilitates adhesion of microbes which then forms biofilms providing a niche for increased survival of organisms. This is intimately associated with infection and management of such biofilm-based catheter-related infection pose a great challenge because of increasing resistance of biofilm-associated micro-organisms to antimicrobial agents. [5] Our study also indicate

increased resistance of biofilm-adherent Candida cells isolated from catheterised urine samples to clinically used antifungal agents. This may explain why antifungal treatment of CAUTI often does not achieve long-term eradication of Candida species. Thus in the present scenario, with increasing numbers of CAUTI caused by biofilm-forming organisms, effective strategies should be adopted to prevent CAUTI.

 
 ~ References Top

1.Kamat US, Ferreira A, Amonkar D, Motghare DD, Kulkarni MS. Epidemiology of hospital acquired urinary tract infections in a medical college hospital in Goa. Indian J Urol 2009;25:76-80.  Back to cited text no. 1
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2.Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase-negative staphylococi to plastic tissue cultures: A quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 1985;22:996-1006.  Back to cited text no. 2
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3.National committee for clinical laboratory standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard. 2 nd ed. M27-A2. National Committee for Clinical Laboratory Standards: Wayne, Pa;2002.  Back to cited text no. 3
    
4.Ramage G, Vande Walle K, Wickes BL, Lopez-Ribot JL. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 2001;45:2475-9.  Back to cited text no. 4
    
5.Mah TF, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 2001;9:34-9.  Back to cited text no. 5
    



 
 
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