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  Table of Contents  
CORRESPONDENCE
Year : 2014  |  Volume : 32  |  Issue : 3  |  Page : 351-352
 

Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system: Few comments


1 Department of Microbiology, Mata Gujri Memorial Medical College, Kishanganj, Bihar, India
2 Department of Microbiology, Radha Gobinda Kar Medical College, Kshudiram Basu Sarani, Kolkata, West Bengal, India
3 Department of Microbiology, Sagar Dutta Medical College, Kamarhati, Kolkata, West Bengal, India
4 Department of Microbiology, Malda Medical College, Malda, West Bengal, India

Date of Submission16-Sep-2013
Date of Acceptance20-Dec-2013
Date of Web Publication10-Jul-2014

Correspondence Address:
A Manna
Department of Microbiology, Malda Medical College, Malda, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.136614

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How to cite this article:
Roy A, Ghosh T, Patra S K, Manna A. Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system: Few comments. Indian J Med Microbiol 2014;32:351-2

How to cite this URL:
Roy A, Ghosh T, Patra S K, Manna A. Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system: Few comments. Indian J Med Microbiol [serial online] 2014 [cited 2019 Sep 19];32:351-2. Available from: http://www.ijmm.org/text.asp?2014/32/3/351/136614


Dear Editor,

We read with great interest the article of Maiti et al., entitled "Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system" in the April 2013 issue of IJMM. [1]

This system appears to be a remarkable modification of the anaerobic bag culture method. [2] We have tried to replicate the described system, in our institutions. We highlight our perception regarding few difficulties we have faced while conducting this experiment.

  • Jar must be absolutely leak-proof, which will be indicated by post-combustion negative pressure created in the jar felt up to the end of the experiment. Alternatively, appropriate indicator tablet should be used
  • Jar lid should be closed gently; otherwise flame may be extinguished early by created air turbulence
  • Regarding technique for preparing stocks of pre-reduced media, the whole process of pre-reduction is to be done at room temperature for 24-48 hours before stocking in the refrigerator. Otherwise the chemical combustion will not be completed at cooler temperature
  • Additional CO 2 generator is not mandatory; being heavier this gas may only help to replace the air inside petri-plate so that the air driven out is made available to react with steel wool more efficiently. Also this will help to fill up the created post-combustion vacuum partially
  • Probably alkaline pyrogallol [3] may not be a good substitute for acidified steel wool because it often produces some amount of carbon monoxide, which is inhibitory to some organisms. Moreover, steel wool has a larger reactant surface, which may lead to faster oxygen purging
  • Aero tolerant study is mandatory to confirm obligate anaerobic growth because some facultative anaerobes may also grow with minimal dissolved oxygen in the media. Most medically important anaerobes, if not all, will grow by this method.


Over-and-all the innovative technique for anaerobiosis is a very cost-effective and simpler method suitable for small to large scale laboratories. The greatest advantage of the technique is that the unavoidable toxic O 2 exposure time during handling, can be reduced substantially due to scope of anaerobic inoculation at collection point. Thus it will meet-up a long-pending need of most laboratories.

 
 ~ References Top

1.Maiti PK, Haldar J, Mukherjee P, Dey R. Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system. Indian J Med Microbiol 2013;31:173-6.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
2.Rosenblatt JE, Stewart PR. Anaerobic bag culture method. J Clin Microbiol 1975;1:527-30.  Back to cited text no. 2
[PUBMED]    
3.Nicol H. Note on anaerobiosis and the use of alkaline solutions of pyrogallol. Biochem J 1929;23:324-6.  Back to cited text no. 3
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