|Year : 2014 | Volume
| Issue : 3 | Page : 349-350
Prevalence and molecular characterisation of metallo-beta-lactamase producing strains of imipenem-resistant Pseudomonas aeruginosa in Turkey
N Ozkalay Yilmaz1, N Agus1, E Bozcal2, A Uzel2
1 Department of Microbiology Laboratory, Tepecik Educational and Research Hospital, Izmir, Turkey
2 Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, Izmir, Turkey
|Date of Web Publication||10-Jul-2014|
N Ozkalay Yilmaz
Department of Microbiology Laboratory, Tepecik Educational and Research Hospital, Izmir
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Yilmaz N O, Agus N, Bozcal E, Uzel A. Prevalence and molecular characterisation of metallo-beta-lactamase producing strains of imipenem-resistant Pseudomonas aeruginosa in Turkey. Indian J Med Microbiol 2014;32:349-50
|How to cite this URL:|
Yilmaz N O, Agus N, Bozcal E, Uzel A. Prevalence and molecular characterisation of metallo-beta-lactamase producing strains of imipenem-resistant Pseudomonas aeruginosa in Turkey. Indian J Med Microbiol [serial online] 2014 [cited 2020 Mar 28];32:349-50. Available from: http://www.ijmm.org/text.asp?2014/32/3/349/136622
Acquired carbapenem resistance due to the production of metallo beta-lactamases (MBLs) has been increasingly reported, particularly for Pseudomonas aeruginosa and Acinetobacter spp. Prompt detection and recognition of the MBLs is important to implement adequate counter-measures to control the spread of the organisms bearing these enzymes, and proper treatment of infections caused by MBL-producing microorganisms. The aim of this study is to identify imipenem (IMP) resistant strains of P. aeruginosa from various clinical specimens of inpatients at our hospital and to investigate the presence of MBL-producing strains among these isolates by phenotypic and genotypic methods and to compare the results with each other.
The study included randomly selected un-duplicate 38 IMP resistant P. aeruginosa isolates from clinical specimens collected in 2010. Isolates were identified by conventional testing and VITEK-2 (bioMerieux, France) automatic identification system. The IMP susceptibility testing was performed by the E-Test (AB Biodisk, Solna, Sweden) according to manufacturer's recommendations. P. aeruginosa ATCC 27853 was used as a control. MBL production of IMP resistant isolates was screened phenotypically by the double disk synergy test (DDST) using an IMP disk (10 μg) and an IMP-EDTA disk (750 μg) on Mueller-Hinton agar (Salubris, Turkey) with 10 mm distance from the edge to the edge of the disk. After overnight incubation, the presence of even a synergistic inhibition zone was interpreted as positive.  IMP-EDTA disks were prepared from commercially available chemicals with 0.5 M EDTA (Sigma Chemical, Germany). Multiplex PCR and subsequent sequencing were carried on in order to identify the blaVIM and blaIMP MBL genes.  PCR amplicons were identified by multiplex PCR were sequenced.
A total of 38 IMP resistant P. aeruginosa strains were analysed. Strains were dominantly isolated from the pus (n: 16, 42.1%), also blood cultures (n: 10, 26.3%), urinary tract (n: 6, 15.8%) and tracheal aspirate specimens (n: 6, 15.8%). Bacterial strains were isolated mainly from intensive care unit (n: 18, 47%), general surgery (n: 12, 32%) and internal medicine (n: 8, 21%). The majority of MBL-producing isolates were isolated from pus and from intensive care unit. MIC 90 values for IMP was found to be 32 μg/ml. Carbapenem resistant isolates were detected concurrently resistant to ciprofloxacin 82%, gentamicin 91% and amikacin 82%. All strains were susceptible to colistin and polimyxin B (MIC 90 values were detected, 0.50 μg/ml for both). The MBL enzyme was found positive in 27 of 38 (71.05%) IMP resistant P. aeruginosa isolates by DDST. Eight of 38 (21.05%) MBL-positive isolates were confirmed to be positive for MBL by PCR [Figure 1]. Nucleotide sequence analysis of the amplicons showed seven strains positive for the bla VIM-2 , one strain positive for the bla IMP-9 gene, which was confirmed by BLAST. No other MBLs were detected. Although 20 false-positive results were found by the MBL-DDST, there was no false-negative result among the isolates of P. aeruginosa [Table 1].
The occurrence of an MBL-positive isolate in a hospital setting poses a therapeutic problem, as well as a serious concern for infection control management. The prevalence of MBL-positive strains among IPM resistant P. aeruginosa isolates from our hospital was estimated at 71.05% (27/38) by DDST and 21% (8/38) by PCR. The prevalence of P. aeruginosa that produce MBL can be markedly different in distinct geographical areas, even among different hospitals in the same area. Our results in this study remind us that metallo enzymes have become a serious clinical and therapeutic problem for our hospital.
The accurate identification and reporting of MBL-producing P. aeruginosa will aid infection control practitioners in preventing the spread of these multidrug-resistant isolates. Many phenotyping methods have performed to search MBL enzymes of P. aeruginosa strains. All these methods are based on the ability of metal chelators, such as EDTA and thiol-based compounds, to inhibit the activity of MBL. The DDST has been reported to be simple, inexpensive phenotypic resources for detection of MBL that could be easily incorporated into the routines of clinical laboratories. , Although there was any false-negative results were detected, 20 false-positive results were found by the MBL-DDST [Table 1]. Chu et al.,  reported that the false positive results in P. aeruginosa by E-test and disk synergy methods were due to the EDTA susceptibility of the strains. It is known that EDTA may increase bacterial cell wall permeability and that zinc (chelated by EDTA) accelerates IPM decomposition and decreases OprD expression of P. aeruginosa.  In this study, not all MBL genes only blaVIM and blaIMP MBL genes were invesitigated. The reason for false positive result in P. aeruginosa by DDTS could be due to this reason.
|Figure 1: Detection of MBL genes (blaVIM and blaIMP) by multiplex PCR. 1. Lane (1-16); M, 4-4, 12-12, A-1, 5-b, 16-16, 1-H, A-9, 8-b, 3-3, 3-b, 18-18, A-4, A-12, A-6, 10-10, 2. Lane (1-4); M, 19-19, A-17, A-20. BlaVIM genes (261 bp): 16-16, 8-b, 3-3, 18-18, A-12, A-6, A-17, BlaIMP gene (765 bp): 1-H|
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|Table 1: PCR positivity among DDSTa positive and negative strains of P. aeruginosa|
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We conclude that synergy-based test results should be confirmed further genetically until standard criteria for performing and evaluating the phenotypic MBL-screening tests are established for EDTA-susceptible and -non-susceptible strains.
| ~ Acknowledgments|| |
This work was partially funded by a grant from the Ege University Scientific Research Foundation Grant No: 2009 Fen 017.
| ~ References|| |
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