|Year : 2014 | Volume
| Issue : 2 | Page : 208-210
Evaluation of dried blood spot as an alternative specimen for the diagnosis of anti-HCV in resource-limited settings
P Nandagopal1, H Syed Iqbal1, S Saravanan1, SS Solomon2, Shruti Mehta3, M Selvakumar1, E Chandrasekhar1, S Solomon1, P Balakrishnan1
1 Infectious diseases Laboratory, YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus Taramani, Chennai, India
2 Johns Hopkins School of Medicine, Baltimore, USA
3 Infectious diseases Laboratory, YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus Taramani, Chennai, India; Johns Hopkins School of Public Health, Baltimore, USA 21205
|Date of Submission||17-May-2013|
|Date of Acceptance||09-Aug-2013|
|Date of Web Publication||2-Apr-2014|
Infectious diseases Laboratory, YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services Hospital Campus Taramani, Chennai, India
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Nandagopal P, Iqbal H S, Saravanan S, Solomon S S, Mehta S, Selvakumar M, Chandrasekhar E, Solomon S, Balakrishnan P. Evaluation of dried blood spot as an alternative specimen for the diagnosis of anti-HCV in resource-limited settings. Indian J Med Microbiol 2014;32:208-10
|How to cite this URL:|
Nandagopal P, Iqbal H S, Saravanan S, Solomon S S, Mehta S, Selvakumar M, Chandrasekhar E, Solomon S, Balakrishnan P. Evaluation of dried blood spot as an alternative specimen for the diagnosis of anti-HCV in resource-limited settings. Indian J Med Microbiol [serial online] 2014 [cited 2020 Jan 24];32:208-10. Available from: http://www.ijmm.org/text.asp?2014/32/2/208/129867
It was estimated that 170 million people are infected with HCV worldwide, with about 3 million new infections every year.  In India, 12-13 million HCV carriers  have been identified so far; thus the diagnosis and characterization of HCV is imperative for the management of HCV disease. The DBS specimen has been found to be the best alternativeto blood derivatives for diagnosing infectious diseases. , The present study optimized and evaluated the use of DBS specimens for anti-HCV antibody testing.
The study was done at YRG Centre for AIDS Research and Education (YRG CARE), Chennai, using DBS and plasma specimens. The DBS specimens (n = 60) were prepared with 903 ® Protein saver card (Whatman, NJ, USA) by absorbing 50 μL whole blood in circles. Two 6-mm diameter discs from each circle of DBS were incubated with 260 μL of specimen diluent overnight at 25-30°C. Two hundred microlitres of DBS elute and parallel plasma specimens were tested for anti-HCV ELISA [Murex Biotech S.A (Pty) Ltd, Kyalami, SA].
There was no difference in qualitative results between plasma and DBS specimens [Table 1]. The sensitivity, specificity, positive predictive value, negative predictive value and efficiency of DBS specimen were found to be 100%. The overall mean OD values of DBS and plasma specimens are 1.041 and 1.439, SD (0.977; 1.31), respectively. The Mann-Whitney U test demonstrated that there was a significant difference in optical density (OD) of these specimens types for both positive (P < 0.01) and negative (P = 0.03) results. The Pearson's correlation coefficient (r) between the specimen types was 0.98 overall and was 0.98 and 0.99 for positive and negative specimens, respectively.
|Table 1: Performance characteristics of DBS specimens to detect anti-HCV antibodies against the conventional plasma specimens|
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DBS specimens make blood collection easier with IDUs, infants and individuals with thrombosed veins, etc., and it does not require centrifugation or temperature-sensitive transport systems. In conclusion, the DBS specimens could be a reliable alternative testing specimen, which may increase HCV diagnosing opportunities for rural, remote and hard to reach regions. Furthermore, DBS specimens will ultimately be helpful for effective surveillance and field level research purposes in resource-limited settings, as the DBS eliminates many logistical and technical limitations.
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