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  Table of Contents  
COMMENTARY
Year : 2014  |  Volume : 32  |  Issue : 2  |  Page : 148
 

PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates


Hainan Medical University, China, Visiting Professor, Faculty of Medicine, University of Nis, Serbia, Adjunct Professor, Joseph Ayobabalola University, Nigeria

Date of Submission02-Oct-2013
Date of Acceptance09-Dec-2013
Date of Web Publication2-Apr-2014

Correspondence Address:
V Wiwanitkit
Hainan Medical University, China, Visiting Professor, Faculty of Medicine, University of Nis, Serbia, Adjunct Professor, Joseph Ayobabalola University
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.129798

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How to cite this article:
Wiwanitkit V. PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates. Indian J Med Microbiol 2014;32:148

How to cite this URL:
Wiwanitkit V. PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates. Indian J Med Microbiol [serial online] 2014 [cited 2019 Nov 22];32:148. Available from: http://www.ijmm.org/text.asp?2014/32/2/148/129798


The genus Acinetobacter comprises many clinical important species. [1] The identification of the problematic species is very important in clinical practice. Within the few years, there is an increased consideration on the clinically important Acinetobacter. According to a recent report from a hospital in India, multi-drug resistant Acinetobacter species (9%) became an uprising important nosocomial pathogen and the rapid diagnosis and prompt treatment is the key for successful clinical management. [2] The advanced molecular biology technique can help discriminate between problematic and non-problematic species, which is better than the classical microbiological technique that has limitation in discrimination. Focusing on the classical technique, the problem of 'unidentification' can be seen and this becomes the reason for the requirement of the new diagnostic technique. [3]

As already mentioned, the applied molecular biology technique can help identify the Acinetobacter species. An important method is the use of polymerase chain reaction (PCR) to detect. The focus on the high conservation of primary and secondary structures within species, ribosomal RNA genes (16S and 23S) is mainly used. [4] According to the report by Garcνa-Arata et al., it is confirmed that 'PCR-amplified 16S and 23S rDNA restriction analysis is both an accurate and rapid method for the identification of Acinetobacter at the DNA group level'. [5] In a more detail, of several PCR-based techniques, the best method is repetitive extragenic palindromic PCR. [6]

Due to the increasing problem of problematic strains of Acinetobacter, the setting of the newly diagnostic tool is very important. The present report in the Indian Journal of Medical Microbiology can be a good example showing advantage of the newly developed PCR tool for identifying newly emerged strain of Acinetobacter clinical isolates. [7] The present report follows the basic theory for setting of a PCR system based on 16S and 23S rDNA internal transcribed spacer (ITS), which is proved to provide accuracy result. In fact, this report shows and implies that the newly developed PCR tool should be available in medical laboratory. This should be considered in any clinical microbiology laboratory. There are some points to be considered in setting of such laboratory including (a) the setting has to follow the standard clinical microbiology practice guideline, (b) standard evaluation of the newly set tool based on diagnostic test evaluation is needed and (c) the cost effectiveness analysis of the newly set tool should also be done. Also, in actual usage, the good quality control covering all phases (pre-, intra- and post-analytical phases) is needed. Finally, the future trend of diagnosis of Acinetobacter species should be mentioned. A more advanced technique, microarray, is the hope for fast detection of the antibiotic resistance. [8]

 
 ~ References Top

1.Towner KJ. Acinetobacter: An old friend, but a new enemy. J Hosp Infect 2009;73:355-63.  Back to cited text no. 1
[PUBMED]    
2.Singh NP, Goyal R, Manchanda V, Das S, Kaur I, Talwar V. Changing trends in bacteriology of burns in the burns unit, Delhi, India. Burns 2003;29:129-32.  Back to cited text no. 2
    
3.Kantor LT, Kominos SD, Yee RB. Identification of nonfermentative gram-negative bacteria in the clinical laboratory. Am J Med Technol 1975;41:3-9.  Back to cited text no. 3
[PUBMED]    
4.Gurtler V, Stanisich VA. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 1996;142:3-16.  Back to cited text no. 4
    
5.García-Arata MI, Gerner-Smidt P, Baquero F, Ibrahim A. PCR-amplified 16S and 23S rDNA restriction analysis for the identification of Acinetobacter strains at the DNA group level. Res Microbiol 1997;148:777-84.  Back to cited text no. 5
    
6.Vila J, Marcos MA, Jimenez de Anta MT. A comparative study of different PCR-based DNA fingerprinting techniques for typing of the Acinetobacter calcoaceticus-A. baumannii complex. J Med Microbiol 1996;44:482-9.  Back to cited text no. 6
    
7.Identification of Acinetobacter clinical isolates by polymerase chain reaction analysis of 16S-23S ribosomal ribonucleic acid internal transcribed spacer. Indian J Med Microbiol 2014.32:149-53.  Back to cited text no. 7
    
8.Coyne S, Guigon G, Courvalin P, Périchon B. Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray. Antimicrob Agents Chemother 2010;54:333-40.  Back to cited text no. 8
    




 

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