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 ORIGINAL ARTICLE
Year : 2014  |  Volume : 32  |  Issue : 2  |  Page : 143-147

Identification of Acinetobacter clinical isolates by polymerase chain reaction analysis of 16S-23S ribosomal ribonucleic acid internal transcribed spacer


1 Department of Biology, College of Science, King Khalid University, Abha 61413, Kingdom of Saudi Arabia
2 Community Family and Medicine Department, College of Medicine, King Khalid University, Abha 61421, Kingdom of Saudi Arabia
3 Department of Community Family & Medicine, Assir Central Hospital Lab, Abha, Kingdom of Saudi Arabia
4 Medical internist, Bashair Hospital, Ministry of Health, Khartoum, Sudan
5 Department of Biology, College of Science, King Khalid University, Abha 61413, Kingdom of Saudi Arabia; Department of Environmental Biotechnology, GEBRI Institute, City for Scientific Research and Technology Applications, New Borg El Arab City, Alexandria, Egypt
6 Department of Clinical Microbiology and Parasitology, College of Medicine, King Khalid University, Abha, Saudi Arabia

Correspondence Address:
MAA Sarhan
Department of Biology, College of Science, King Khalid University, Abha 61413, Kingdom of Saudi Arabia

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Source of Support: Grant (KKU-MED-11-017) from the Deanship of scientifi c research, King Khalid University, Abha, Saudi Arabia,, Conflict of Interest: None


DOI: 10.4103/0255-0857.129797

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Background: The genus Acinetobacter is a diverse group of Gram-negative bacteria involve at least 33 species using the molecular methods. Although the genus Acinetobacter comprises a number of definite bacterial species, some of these species are of clinical importance. Therefore, it is of vital importance to use a method which is able to reliably and efficiently differentiate the numerous Acinetobacter species. Objectives: This study aims to identify Acinetobacter of clinical isolates from Assir region to the species level by 16S-23S intergenic spacers internal transcribed spacer (ITS) of ribosomal ribonucleic acid (rRNA). Materials and Methods: Deoxyribonucleic acid extraction, polymerase chain reaction amplification of 16S-23S intergenic spacer sequences (ITS) was performed using the bacterium-specific universal primers. Results: Based on the 16S-23S intergenic spacers (ITS) of rRNA sequences, all isolates tested were identified as Acinetobacter baumannii. The isolates shared a common ancestral lineage with the prototypes A. baumannii U60279 and U60280 with 99% sequence similarities. Conclusion: These findings confirmed 16S-23S rRNA ITS for the identification of A. baumannii of different genotypes among patients.






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