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Year : 2013  |  Volume : 31  |  Issue : 2  |  Page : 173-176

Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system


Department of Microbiology, Institute of Post Graduate Medical Education and Research, AJC Bose Road, Kolkata, India

Correspondence Address:
P K Maiti
Department of Microbiology, Institute of Post Graduate Medical Education and Research, AJC Bose Road, Kolkata
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.115218

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Success for maximum isolation of anaerobes depends on maintaining critically low oxygen levels throughout and growth in a reduced medium with exclusion of inhibitory substances. Hence a dual system was used equipped with candle combustion for instant exhaustion of major part of oxygen from a sealed jar, along with acidified steel wool for residual oxygen purging. For inhibitory substances removal, test anaerobes were grown on anaerobic medium layered on buffer charcoal agar bed. After 48 hours incubation average colony sizes were compared with that of growths in conventional Gas-Pak system. Better growths were noted in the innovative system.






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