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 ORIGINAL ARTICLE
Year : 2013  |  Volume : 31  |  Issue : 2  |  Page : 148-153

Intraperitoneal inoculation of Haemophilus influenzae local isolates in BALB/c mice model in the presence and absence of virulence enhancement agents


1 Biotechnology Department, Iran Reference Health Lab, Ministry of Health and Medical Education, and Pathology, Milad National Hospital, Tehran, Iran
2 Department of Quality Control, Razi Vaccine and Serum Research Institute, Karadj, Iran
3 Department of Microbiology, Iran Reference Health Lab, Ministry of Health and Medical Education, and Pathology, Milad National Hospital, Tehran, Iran
4 Department of Medical Vaccine Research, Razi Vaccine and Serum Research Institute, Karadj, Iran
5 Department of Pathology, Razi Vaccine and Serum Research Institute, Karadj, Iran

Correspondence Address:
N Mojgani
Biotechnology Department, Iran Reference Health Lab, Ministry of Health and Medical Education, and Pathology, Milad National Hospital, Tehran
Iran
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Source of Support: Ministry of Jihad-e-agriculture Iran (project No 2-18-18-88038), Conflict of Interest: None


DOI: 10.4103/0255-0857.115236

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Purpose:Haemophilus influenzae (Hi), predominantly type b accounts for approximately 4% of cases of community-acquired and nosocomial meningitis, in adults. The objective of this study was to evaluate the pathogenicity of local Hi isolates (type b, f and non-typable) in BALB/c mice in the presence of virulence enhancement agents. Materials and Methods: Three different concentrations of the Hi isolates were inoculated intraperitoneally in BALB/c mice in the presence of 2% hemoglobin and 4% mucin as virulence enhancing agents (VEA). The ability of the isolates to produce bacteremia, the percent survival and lethal dose (LD 50 ) were recorded in different challenge groups. Results: The 3 Haemophilus influenzae type b (Hib) isolates used in study were able to show virulence in BALB/c mice model only in the presence of VEA and their LD 50 decreased significantly when 2% hemoglobin and 4% mucin were used. All survived animals showed bacteremia within 4 h of inoculation which was cleared within 18 h. Significant differences ( P < 0.01) in the virulence and survival percentage of Hib challenge groups were observed based on their dose of inoculation and VEA. None of the isolates were able to induce infection in the absence of VEA. Non-type b isolates failed to produce disease in the mice models even at the highest inoculated dose (10 8 cfu) and in the presence of VEA. Conclusions: BALB/c mice appeared suitable for evaluating the virulence of Hib strains, and 2% hemoglobin with 4% mucin an appropriate concentration for inducing infection in this animal model.






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