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 ORIGINAL ARTICLE
Year : 2013  |  Volume : 31  |  Issue : 2  |  Page : 142-147

Detection of the common resistance genes in Gram-negative bacteria using gene chip technology


Clinical Research Center of Ningbo No. 2 Hospital, Ningbo, Zhejiang, 315010, China

Correspondence Address:
Z Shun
Clinical Research Center of Ningbo No. 2 Hospital, Ningbo, Zhejiang, 315010
China
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Source of Support: This work was fi nancially supported by Clinical research center of Ningbo No.2 Hospital, Conflict of Interest: None


DOI: 10.4103/0255-0857.115230

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Objective: To design a resistance gene detection chip that could, in parallel, detect common clinical drug resistance genes of Gram-negative bacteria. Materials and Methods: Seventy clinically significant Gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii) were collected. According to the known resistance gene sequences, we designed and synthesized primers and probes, which were used to prepare resistance gene detection chips, and finally we hybridized and scanned the gene detection chips. Results: The results between the gene chip and polymerase chain reaction (PCR) were compared. The rate was consistently 100% in the eight kinds of resistance genes tested (TEM, SHV, CTX-M, DHA, CIT, VIM, KPC, OXA-23). One strain of Pseudomonas aeruginosa had the IMP, but it was not found by gene chip. Conclusion: The design of Gram-negative bacteria-resistant gene detection chip had better application value.






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2004 - Indian Journal of Medical Microbiology
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