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  Table of Contents  
CORRESPONDENCE
Year : 2013  |  Volume : 31  |  Issue : 1  |  Page : 92
 

Corrections in molecular characterization of S. aureus


Clinical Associate Professor, Microbiology, Amrita Institute of Medical Sciences, Kochi, Kerala, India

Date of Submission07-Sep-2012
Date of Acceptance06-Nov-2012
Date of Web Publication15-Mar-2013

Correspondence Address:
V A Kumar
Clinical Associate Professor, Microbiology, Amrita Institute of Medical Sciences, Kochi, Kerala
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.108748

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How to cite this article:
Kumar V A. Corrections in molecular characterization of S. aureus. Indian J Med Microbiol 2013;31:92

How to cite this URL:
Kumar V A. Corrections in molecular characterization of S. aureus. Indian J Med Microbiol [serial online] 2013 [cited 2019 Aug 18];31:92. Available from: http://www.ijmm.org/text.asp?2013/31/1/92/108748


Dear Editor,

The authors have done an excellent job of determining the presence of PVL and mecA genes in S. aureus isolates and also typing the SCCmec element. [1] However, few details need correction.

  • It is not clear as to how the authors phenotypically screened MRSA, i.e., using oxacillin, methicillin, or cefoxitin.
  • The original study by Zang et al. used nine pairs of primers for SCC mec typing. [2] The authors have used the same protocol but missed mentioning the mecA internal control in the primer list and Materials and Methods section.
  • The authors have used the multiplex protocol of Jo-Ann McClure et al. for 16SrRNA and PVL detection and have wrongly mentioned that the 16SrRNA is specific for S. aureus. The primers used for 16SrRNA are genus specific and will be amplified even for S. epidermidis.[3]
  • Augumentin is the trade name, therefore, amoxicillin/clavulanic acid should have been used instead.


 
 ~ References Top

1.Moussa I, Kabli SA, Hemeg HA, Al-Garni M, Shibl AM. A novel multiplex PCR for molecular characterization f methicillin resistant Staphylococcus aureus recovered from Jeddah, Kingdom of Saudi Arabia. Indian J Med Microbiol 2012;30:296-301.  Back to cited text no. 1
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2.Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2005;43:5026-33.  Back to cited text no. 2
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3.McClure JA, Conly JM, Lau V, Elsayed S, Louie T, Hutchins W, et al. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. J Clin Microbiol 2006;44:1141-4.  Back to cited text no. 3
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