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 ORIGINAL ARTICLE
Year : 2012  |  Volume : 30  |  Issue : 4  |  Page : 391-396

Development and evaluation of reverse-transcription loop-mediated isothermal amplification for rapid detection of human immunodeficiency virus type 1


1 Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
2 Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China
3 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
4 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640; Guangzhou Wondfo Biotech Co., Ltd, Guangzhou 510530, China
5 Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, China
6 Center for Disease Control and Prevention of Guangdong Province, Institute of Public Health, Guangzhou 510030, China
7 Guangzhou Wondfo Biotech Co., Ltd, Guangzhou 510530, China

Correspondence Address:
Xihong ZHAO
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640
China
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Source of Support: Research Fund of Wuhan Institute of Technology (10113201), China; National Natural Science Foundation of China (81172178), Conflict of Interest: None


DOI: 10.4103/0255-0857.103757

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Purpose: The objective of this study was to establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of human immunodeficiency virus type 1 (HIV-1). Materials and Methods: The HIV-1 integrase gene region was selected because it was a conserved part of the HIV-1 genome. Six primers specific to eight regions of the HIV-1 integrase gene were designed. A total of 171 samples (18 HIV-1 confirmed positive samples and 153 serum specimens were collected in this study) were tested by RT-LAMP and reverse-transcription polymerase chain reaction (RT-PCR). After amplification in an isothermal water bath for 45 min, samples containing HIV-1 generated the expected ladder-like products while other viruses generated no product. Results: The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with RT-PCR. The assay was significantly more sensitive than normal gel-based RT-PCR. Conclusion: Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of HIV-1.






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