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CORRESPONDENCE
Year : 2012  |  Volume : 30  |  Issue : 3  |  Page : 374
 

Recent updates on Methicillin resistant Staphylococcus aureus typing


Department of Microbiology, Amrita Institute of Medical Sciences, Ponekara, Kochi 682041, Kerala, India

Date of Submission02-Apr-2012
Date of Acceptance07-Apr-2012
Date of Web Publication8-Aug-2012

Correspondence Address:
V A Kumar
Department of Microbiology, Amrita Institute of Medical Sciences, Ponekara, Kochi 682041, Kerala
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.99513

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How to cite this article:
Kumar V A. Recent updates on Methicillin resistant Staphylococcus aureus typing. Indian J Med Microbiol 2012;30:374

How to cite this URL:
Kumar V A. Recent updates on Methicillin resistant Staphylococcus aureus typing. Indian J Med Microbiol [serial online] 2012 [cited 2019 Aug 20];30:374. Available from: http://www.ijmm.org/text.asp?2012/30/3/374/99513


Dear Editor,

The article "Typing of Methicillin resistant Staphylococcus aureus: A technical review" by Mehndiratta et al.[1] was very informative but missed out two new methods that have been recently reported.

  1. SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) has been evaluated to investigate outbreaks and was found to be simple, reproducible and highly discriminatory without the need of expensive equipment or specialist expertise and has the potential to be used in routine clinical microbiology laboratories. [2]
  2. A novel application using denaturing HPLC (DHPLC) utilizing the variability within the staphylococcal protein A, or spa A, gene-repeat region as a marker of short- and long-term genetic variation for rapid, inexpensive characterization of spaA gene amplification products, without the need for DNA sequence determination has been described lately. Within 5 h of from receipt of a primary isolation sub-typing of isolates was feasible and moreover relatedness could be demonstrated 5 days before results were received from a reference laboratory. [3]


 
 ~ References Top

1.Mehndiratta PL, Bhalla P. Typing of Methicillin resistant Staphylococcus aureus: A technical review. Indian J Med Microbiol 2012;30:16-23.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
2.Al-Zahrani IA, Hamson C, Edge D, Collins J, Perry JD, Raza M, et al. Smal restriction site-based multiplex PCR for typing of hospital- and community-acquired Staphylococcus aureus. J Clin Microbiol 2011;49:3820-8.  Back to cited text no. 2
[PUBMED]    
3.Jury F, Al-Mahrous M, Apostolou M, Sandiford S, Fox A, Ollier W, et al. Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC. J Med Microbiol 2006;55:1053-60.  Back to cited text no. 3
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2004 - Indian Journal of Medical Microbiology
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