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Year : 2012  |  Volume : 30  |  Issue : 3  |  Page : 342-345

Rapid culture diagnosis of tuberculous lymphadenitis from a tertiary care centre in an endemic nation: Potential and pitfalls


1 Department of Microbiology, VMMC and Safdarjung Hospital, 110 029, India
2 Department of Histopathology, VMMC and Safdarjung Hospital, 110 029, India
3 Department of Bio-Sciences, Jamia Millia Islamia University, 110 025, New Delhi, India

Correspondence Address:
D Kasana
Department of Microbiology, VMMC and Safdarjung Hospital, 110 029
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.99498

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In spite of low sensitivity and specificity, standard diagnostic algorithm recommends fine needle aspiration cytology (FNAC) and direct microscopic screening for acid-fast bacilli (AFB) for the routine diagnosis of tuberculous lymphadenopathy (LNTB). In this study, the diagnostic utility of liquid broth based automated culture (BacT/ALERT 3D) technique was assessed in comparison with conventional techniques in 89 clinically suspected tubercular lymphadenitis patients. 60% (n = 53) were positive by FNAC and 38.4% (n = 34) demonstrated AFB in smear examination. BacT/ALERT yielded isolation in 43.1% (n = 38) aspirates, confirming tubercular aetiology. We also found six paediatric culture-positive cases which showed negative outcome by both FNAC and smear. Thus, we conclude that culture by BacT/ALERT, may be used for faster yield of Mycobacteria in LNTB, especially in children. Additionally, this could also be used as a platform for further differentiation of Mycobacterium tuberculosis from non-tuberculous mycobacteria (NTM) infection and for testing of anti-tubercular chemotherapeutic agents whenever drug resistance is suspected






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2004 - Indian Journal of Medical Microbiology
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