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 ORIGINAL ARTICLE
Year : 2012  |  Volume : 30  |  Issue : 2  |  Page : 175-181

Molecular screening of virulence genes in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in Northwest Iran


1 Research Center of Infectious Diseases and Tropical Medicine; Faculty of Medicine Department of Clinical Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran
2 Faculty of Medicine Department of Clinical Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran
3 Research Center of Infectious Diseases and Tropical Medicine, Department of Infectious Diseases, Tabriz University of Medical Sciences, Tabriz, Iran
4 Research Center of Infectious Diseases and Tropical Medicine; Department of Clinical Biochemistry and Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
5 Faculty of Medicine Department of Clinical Microbiology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
6 Research Center of Infectious Diseases and Tropical Medicine, Tabriz University of Medical Sciences, Tabriz, Tabriz, Iran

Correspondence Address:
Y Sharifi
Research Center of Infectious Diseases and Tropical Medicine; Faculty of Medicine Department of Clinical Microbiology, Tabriz University of Medical Sciences, Tabriz
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.96687

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Purpose: The present study screened clinical isolates of Enterococcus faecalis and Enterococcus faecium to determine the prevalence of high-level gentamicin-resistant enterococci and the potential virulence genes among them. Materials and Methods: Clinical enterococcal isolates were obtained from three university teaching hospitals in Northwest Iran. Isolated enterococci were identified phenotypically followed by antibiotic susceptibility testing. Multiplex PCR was performed for the detection of genus, species-specific targets, gentamicin resistance, and potential virulence genes. Results: Of 220 enterococcal isolates, 133 (60.45%) isolates were identified as high-level gentamicin-resistant. Of these isolates, 79 (59.4%) and 54 (40.6%) were E. faecalis and E. faecium, respectively. All high-level gentamicin-resistant strains carried aac(6′)Ie-aph(2″)Ia. Of 220 isolates, 65.9% were positive for gelE, and 55%, 53.6%, 51.8%, and 49.5% of isolates were positive for cpd, asa1, ace, and esp, respectively. Phenotypically detected β-haemolytic strains (19.54%) were found to possess cylL ls MAB. Conclusion: The study revealed that high-level gentamicin-resistance was related to the presence of aac(6′)Ie-aph(2″)Ia. Isolated enterococci harboured potential virulence determinants, which were more common among E. faecalis than among E. faecium strains.






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