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 ORIGINAL ARTICLE
Year : 2012  |  Volume : 30  |  Issue : 1  |  Page : 34-38

In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin against Pseudomonas aeruginosa


Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, 34116 Beyazit, Istanbul, Turkey

Correspondence Address:
C Bozkurt-Guzel
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, 34116 Beyazit, Istanbul
Turkey
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Source of Support: None, Conflict of Interest: None


PMID: 22361758

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Purpose: In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin were investigated by studying time-kill kinetics and post-antibiotic effect (PAE) against strains of Pseudomonas aeruginosa isolated from patients with cystic fibrosis. Method: Synergy was investigated at 0.5×, 1× and 5× MIC of antibiotics using time-kill curve method. PAEs were determined by the standard viable counting method where bacteria in the logarithmic phase of growth were exposed for 1 h to the antibiotics at 1× or 20× MIC, alone and in combinations. Synergy and additive effects were detected at 1×MIC, at 24 h. Results: Some of the strains produced an earlier synergistic effect at 12 h. No antagonism was observed. Colistin methanesulfonate and amikacin produced PAEs 1.16 ± 0.10 to 2.25 ± 0.16 h and 0.96 ± 0.15 to 2.69±0.32 h, respectively. When the antibiotics were used in combination the PAEs were prolonged to a value of 3.88±0.25 h. Consequently, the Conclusions: Findings of this study may play useful role in selecting the appropriate combinations when a single agent is inadequate, and may have important information for optimizing the dose intervals.






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2004 - Indian Journal of Medical Microbiology
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