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 ORIGINAL ARTICLE
Year : 2011  |  Volume : 29  |  Issue : 3  |  Page : 258-261

First detection of TEM-116 extended-spectrum β-lactamase in a Providencia stuartii isolate from a Tunisian hospital


1 laboratory of microbiology, Department of biology, Military Hospital of Tunis, Tunis, Tunisia
2 Infections with Multiresistant Bacteria to Antibiotics (UR/29/04), Department of Microbiology, University Hospital of Sahloul, Sousse, Tunisia
3 Laboratory of Biochemistry and Biotechnology, Department of biology, Faculty of Sciences of Tunis, Campus Universitaire, Tunis, Tunisia

Correspondence Address:
H Lahlaoui
laboratory of microbiology, Department of biology, Military Hospital of Tunis, Tunis
Tunisia
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Source of Support: Tunisian Ministry of Higher Education, Scientifi c Research and Technology, Conflict of Interest: None


DOI: 10.4103/0255-0857.83909

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Purpose: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes Materials and Methods: This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. Results: This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase. Conclusion: This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.






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2004 - Indian Journal of Medical Microbiology
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