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CORRESPONDENCE
Year : 2011  |  Volume : 29  |  Issue : 2  |  Page : 194-195
 

A pilot cross-sectional study to determine the utility of an oral HIV1/2 point of care test on sputum for screening TB/HIV co-infection in Central India


1 Department of Pathology, Bhopal Memorial Hospital and Research Centre, Bhopal, India
2 Department of Pulmonary Medicine, McGill University, Royal Victoria Hospital, McGill University Health Centre (MUHC), Montreal, QC, Canada
3 Department of Microbiology, Bhopal Memorial Hospital and Research Centre, Bhopal, India

Date of Submission10-Dec-2010
Date of Acceptance17-Dec-2010
Date of Web Publication2-Jun-2011

Correspondence Address:
P Desikan
Department of Pathology, Bhopal Memorial Hospital and Research Centre, Bhopal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.81781

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How to cite this article:
Desikan P, De S, Pai N P, Panwalkar N, Verma M, Jain A. A pilot cross-sectional study to determine the utility of an oral HIV1/2 point of care test on sputum for screening TB/HIV co-infection in Central India. Indian J Med Microbiol 2011;29:194-5

How to cite this URL:
Desikan P, De S, Pai N P, Panwalkar N, Verma M, Jain A. A pilot cross-sectional study to determine the utility of an oral HIV1/2 point of care test on sputum for screening TB/HIV co-infection in Central India. Indian J Med Microbiol [serial online] 2011 [cited 2019 Dec 15];29:194-5. Available from: http://www.ijmm.org/text.asp?2011/29/2/194/81781


Dear Editor,

The OraQuick® HIV-1/2 assay (OraSure Technologies, Inc., Bethlehem, PA, USA) is a noninvasive, point-of-care, simple, convenient, visually read qualitative immunochromatographic test for the detection of antibodies to HIV-1 and -2 in gingival secretions and blood. Since the OraQuick assay on sputum samples has not been extensively evaluated in India, we attempted to examine the diagnostic performance and client preference for OraQuick assay on sputum for screening TB/HIV co-infected populations in Central India.

Seventy-one adult participants suspected to be co-infected with HIV/TB were enrolled in the study. Sputum specimens, either smear positive or culture positive for Mycobacterium tuberculosis, were included. Sputum samples from patients who were culture positive were collected at follow-up visits for HIV testing with OraQuick HIV-1/2 assay. The participants were provided pre-test and post-test counselling as per the guidelines of the National AIDS Control Organization (NACO), India. HIV antibody testing was conducted on sputum specimens using OraQuick HIV-1/2 assay, followed by confirmatory testing by dual ELISAs. The OraQuick HIV-1/2 assay in sputum specimens was found to have 100% sensitivity (2/2) and 100% specificity (69/69) [Table 1]. The OraQuick HIV-1/2 assay on sputum was preferred by 100% of the participants over ELISA.
Table 1: Performance of OraQuick® HIV-1/2 assay on sputum specimens from tuberculosis suspects compared with serum ELISA

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Our hospital-based pilot study indicated a good diagnostic accuracy of the Oraquick test on sputum. This testing method may therefore be useful for conducting HIV surveillance in possible co-infected TB patients on sputum specimens collected as a part of the Revised National Tuberculosis Control Program. However, previous studies have reported varying levels of sensitivity and specificity. [1],[2] Hence, extensive community-based estimates are urgently needed to determine the performance of OraQuick for screening HIV infection in TB patients, particularly in view of the recent report of false-positive results occurring in a performance study of OraQuick oral-fluid rapid HIV tests in Minnesota. [3]

 
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1.Grandin W, Dev AV, Latha A, Armstrong L, Mathai D, John KR, et al Detection of human immunodeficiency virus infection in the sputum of tuberculosis patients in South India. Int J Tuberc Lung Dis 2010;14:1288-94.   Back to cited text no. 1
    
2.Talbot EA, Hone NM, Moffat HJ, Lee EJ, Moeti TL, Mokobela K, et al. The validity of HIV testing using sputum from suspected tuberculosis patients in Bostswana, 2001. Int J Tuberc Lung Dis 2003;7:710-3.  Back to cited text no. 2
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3.Jafa K, Patel P, Mackellar DA, Sullivan PS, Delaney KP, Sides TL, et al. OraQuick Study Group. Investigation of false positive results with an oral fluid rapid HIV-1/2 antibody test. PLoS One 2007;31;2:e185.  Back to cited text no. 3
    



 
 
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1 An HIV1/2 point of care test on sputum for screening TB/HIV co-infection in central India Will it work?
Prabha Desikan,Sajal De,Nitika Pant Pai,Pradyumna K Mishra,Kaushal Kumar,Nikita Panwalkar,Mayanka Verma,Zia Ul Hasan,Kewal K Maudar
Asian Pacific Journal of Tropical Medicine. 2013; 6(3): 216
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