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Year : 2011  |  Volume : 29  |  Issue : 2  |  Page : 118-123

High frequency of integrons related to drug-resistance in clinical isolates of Acinetobacter baumannii

1 Department of Laboratory Medicine, Changzhou Tumor Hospital Soochow University, Changzhou 213001, China
2 Department of Oncological Medicine, Changzhou Tumor Hospital Soochow University, Changzhou 213001, China
3 Department of Laboratory Medicine, Changzhou Tumor Hospital Soochow University, Changzhou 213001; Shanghai Key Laboratory of Tuberculosis, The Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China

Correspondence Address:
D Chun-Lei
Department of Laboratory Medicine, Changzhou Tumor Hospital Soochow University, Changzhou 213001
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.81784

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Purpose: As an opportunistic pathogen, Acinetobacter baumannii causes various nosocomial infections. In recent years, the increasing cumulative infection outbreaks involving A. baumannii have appeared worldwide. In addition, a perplexing trouble for clinical treatment is a severe drug-resistance problem with A. baumannii. In this study, we investigated the drug-resistance rates and integrons' distribution in A. baumannii clinical strains in East China. Furthermore, we explored the relationship between integrons and drug resistance. Materials and Methods: Strains were identified using non-fermenting bacteria identification cards by Vitek-32 system. Disk-diffusion method (Kirby-Bauer) was used to judge antimicrobial sensitivity. Integrons and the gene cassettes of integrons were identified by PCR, restriction enzyme digestion and DNA sequencing. Results: Except imipenem and cefoperazone/sulbactam, the drug-resistance rates of the A. baumannii clinical isolates to other 15 kinds of antibacterials, all surpassed 30%. Of 96 A. baumannii clinical isolates, 66 strains carried class 1 integrons (no class 2 or 3 integrons were found). Overall, the drug-resistance rates in integrons-positive A. baumannii to 14 kinds of antibacterials were higher than those in integrons-negative A. baumannii. Gene sequencing showed that 9 of 12 integrons contained seven different gene cassettes (aacA4, catB3, dfrA1, blam-1, orfX, aadA1, and sat2). The cassette arrays aacA4-catB3-dfrA1 was found in five detected integrons. Conclusions: High resistances in A. baumannii clinical strains to most common antimicrobial agents have appeared in East China, which was closely related with high frequencies class 1 integrons. A. baumannii integrons cassettes carried multi-drug-resistant gene codes. We believe that integrons cassettes gene could be taken as a marker of prognosticating A. baumannii antimicrobial resistance, but only reveal partial drug resistance profiles.


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