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 ORIGINAL ARTICLE
Year : 2011  |  Volume : 29  |  Issue : 1  |  Page : 19-21

BinaxNOW® - An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction


HPA West Midlands Public Health Laboratory, Birmingham Heartlands Hospital, United Kingdom

Correspondence Address:
H Osman
HPA West Midlands Public Health Laboratory, Birmingham Heartlands Hospital
United Kingdom
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.76518

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Purpose: A rapid test for influenza viruses (Binax NOW® ) was evaluated. Materials and Methods: In season-1, 35 respiratory samples were tested retrospectively; in season-2, 45 samples were tested prospectively [gold-standard: viral culture in season-1, culture+ reverse transcriptase-polymerase chain reaction (RT-PCR) in season-2]. Results: Sensitivity for Binax for influenza A was 59.3 and 0% in season-1 and -2, respectively. Sensitivity was low for influenza B (33.3% in season-1, 26.1% in season-2). Samples having low viral load were more likely to have a negative Binax test. Specificity of Binax was high (100 and 94.7% with influenza A and B, respectively). Conclusion: Sensitivity information provided in the kit insert does not always reflect post licensure performance in clinical settings.






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