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Year : 2010  |  Volume : 28  |  Issue : 4  |  Page : 376-379

Comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases

1 Department of Microbiology, Dr. HSJ Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India
2 Department of Medical Microbiology, PGIMER, Chandigarh, India
3 Department of Experimental Medicine (SG), PGIMER, Chandigarh, India

Correspondence Address:
J Sharma
Department of Microbiology, Dr. HSJ Institute of Dental Sciences and Hospital, Panjab University, Chandigarh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.71829

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Extended spectrum β-lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. Threat posed by antibiotic resistance because of ESBLs is more serious as a number of technical problems are associated with the detection of these enzymes. Although a number of detection methods have been designed for ESBLs, every method has its own benefits and shortcomings as well. In earlier days, isoelectric focusing (IEF) was used as the gold standard for ESBL detection. This study was undertaken to compare IEF with polymerase chain reaction, a method which has been extensively used for ESBL detection these days.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04