|Year : 2010 | Volume
| Issue : 4 | Page : 370-371
The effect of tigecycline and ertapenem against clinical isolates of Brucella melitensis detected by E-test on different media
E Tanyel1, AY Coban2, N Tasdelen Fisgin1, N Tulek1
1 Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University, Medical School, Samsun, Turkey
2 Department of Microbiology and Clinical Microbiology, Ondokuz Mayis University, Medical School, Samsun, Turkey
|Date of Submission||21-Dec-2009|
|Date of Acceptance||01-Jul-2010|
|Date of Web Publication||20-Oct-2010|
Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University, Medical School, Samsun
Source of Support: None, Conflict of Interest: None
In this study, in vitro activity of tigecycline (TIG) and ertapenem (ERT) against clinical isolates of Brucella melitensis and the effect of different media on in vitro test results were investigated. The in vitro effects of TIG and ERT to 38 B. melitensis isolates were comparatively investigated in brucella agar and 5% sheep blood agar. MIC value of ERT was 0.032 μg/mL in 23 of 38 and 20 of 38 isolates on blood and brucella agar, respectively. Minimum inhibitory concentration values of TIG were substantially different ranging between 0.064-0.25 μg/mL on blood agar. However, MIC values of TIG were similar on brucella agar with 0.25 μg/mL in 15 of 38 isolates and 0.5 μg/mL in 10 of 38 isolates. In conclusion, although ERT and TIG were effective against B. melitensis isolates in vitro, further studies are needed in order to determine the use of these novel drugs in treatment of brucellosis.
Keywords: Brucellosis, tigecycline, ertapenem
|How to cite this article:|
Tanyel E, Coban A Y, Fisgin N T, Tulek N. The effect of tigecycline and ertapenem against clinical isolates of Brucella melitensis detected by E-test on different media. Indian J Med Microbiol 2010;28:370-1
|How to cite this URL:|
Tanyel E, Coban A Y, Fisgin N T, Tulek N. The effect of tigecycline and ertapenem against clinical isolates of Brucella melitensis detected by E-test on different media. Indian J Med Microbiol [serial online] 2010 [cited 2019 Jun 17];28:370-1. Available from: http://www.ijmm.org/text.asp?2010/28/4/370/71822
| ~ Introduction|| |
Brucellosis More Details is a disease that is widely seen especially in the developing countries. Because active microorganism is located inside the cell, it is difficult to treat this disease; combined and long-term antibiotic treatment must be applied. In addition, the drugs that are used should be transferred into the cells successfully and keep their effectiveness in this acidic environment.  Ertapenem (ERT), which is a new antibiotic in our country is a synthetic, long-term effective carbapenem that can be intravenously and intramuscularly applied once a day. Tigecycline (TIG) is a synthetic derivation of minocycline of glycylcycline class. , The glycylcyclines inhibits bacterial protein synthesis by binding ribosomal A zones five times stronger than tetracyclines. In this study, we aimed to investigate the effects of ERT and TIG against Brucella More Details melitensis isolates.
The susceptibilities to TIG and ERT were determined by using E-test (AB Biodisk, Solna, Sweden) method on 5% sheep blood agar (bioMθrieoux SA, Marcy I'Etoile, France) and Brucella agar (Oxoid Ltd., Basingstoke, Hampshire, England) against B. melitensis 38 clinical isolates. The minimum inhibitory concentrations (MICs) of TIG and ERT for all isolates on two blood agar media is summarized in [Table 1]. The MIC values of ERT and TIG were showing differences depending on two blood agar media but there was a marked difference for TIG, and MICs of TIG were higher in brucella agar.
In the in vitro studies that were conducted in order to find the effectiveness of TIG in the brucella strains; it was thought that TIG could be an alternative agent. Dizbay et al.  found that TIG had the lowest values in MIC 50 (0.064 μg/ml) and MIC 90 (0.094 μg/ml) against B. melitensis 16 isolates. In addition, Kilic et al.  determined that TIG had the second lowest level of MIC 90 (0.125 μg/ml) after levofloxacin, and could be an alternative agent in the treatment of brucellosis. Turan et al.  noted that the MIC 90 value of TIG as 0.125 μg/ml was lower than the MIC values that of rifampin and ciprofloxacin. In our study, MIC 90 value of TIG was found to be 0.38 and 1 μg/ml for blood and brucella agar, respectively. In PUBMED scanning, no study about the effectiveness of ERT to the brucella strains was found. MIC 90 value of ERT was found to be 0.047 and 0.064 μg/ml for blood and brucella agar, respectively, in this study.
The CLSI recommends brucella broth whose pH was adjusted to 7.1 ± 0.1 for susceptibility testing.  Therefore, in our study, we used brucella and blood agar media, and we observed that brucella agar is enough for bacterial reproduction. The variability in the results of the conducted studies may be due to the use of different media. In this study, we detected that MIC values were changing according to the media especially for TIG. As a result, it was proved that ERT and TIG are in vitro effective against the B. melitensis isolates. This is the first in vitro study that shows effectiveness of ERT on the brucella strains. There may be alternative drugs in the treatment but in vivo studies are needed in order to use these drugs in treatment of the disease.
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