Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 1295 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~Related articles
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed11740    
    Printed419    
    Emailed17    
    PDF Downloaded1317    
    Comments [Add]    
    Cited by others 5    

Recommend this journal

 

 BRIEF COMMUNICATION
Year : 2010  |  Volume : 28  |  Issue : 3  |  Page : 241-244

Detection and characterization of metallo beta lactamases producing Pseudomonas aeruginosa


1 Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation (BMPLIII), Department of Medicine Unit I and Infectious Diseases, Christian Medical College, Vellore -632004, Tamil Nadu, India
2 Surveillance of Antimicrobial Resistance in India Study Group (SARISG), Kothari Medical Center, Kolkata - Dr. Anuradha Aggarwal; Mahatma Gandhi Institute of Medical Sciences, Wardha - Dr. Vijayshree Deotale & Dr. D. K. Mendiratta; P D Hinduja Hospital, Mumbai - Dr. Camilla Rodriguez; Sri Ramachandra Medical College, Chennai - Dr. Padma Srikanth; Sir Gangaram Hospital, New Delhi - Dr. Chand Wattal; Sundaram Medical Center, Chennai - Dr. Vaidehi & Dr. Ram Rajagopal; Tata Memorial Center, Mumbai - Dr. Rohini Kelkar, India

Correspondence Address:
A Manoharan
Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation (BMPLIII), Department of Medicine Unit I and Infectious Diseases, Christian Medical College, Vellore -632004, Tamil Nadu
India
Login to access the Email id

Source of Support: CIPLA India, Conflict of Interest: None


DOI: 10.4103/0255-0857.66486

Rights and Permissions

This study was undertaken to evaluate phenotypic and genotypic methods for detection of Metallo-Beta-Lactamases (MBLs) among nosocomial Pseudomonas aeruginosa. Sixty one among 176 P. aeruginosa isolates, collected as part of a multicentric study (2005-2007), were evaluated for carbapenem resistance (CARB-R; resistant to either imipenem/meropenem) and screened for MBL by Combination Disk Diffusion Test (CDDT) using imipenem (IMP), meropenem (MER) and ceftazidime (CAZ) with EDTA. MBL positives were further confirmed by IMP + EDTA Etest. Twenty strains (42.6%) were found to be MBL producers among the 61 P. aeruginosa. PCR for IMP and VIM MBL was performed on 48 of the 61, 15 were positive for VIM MBL type. CDDT using IMP + EDTA had the highest sensitivity and specificity of 87.8% and 84.4% when compared to Etest, which was higher than the values obtained for CAZ + EDTA and MER + EDTA. CDDT using IMP + EDTA also compared very well with the PCR (specificity = 90.9%, sensitivity = 93.3%). CARB-R among P. aeruginosa is mediated predominantly via MBL production. Clinical P. aeruginosa isolates can be screened routinely using the less expensive IMP + EDTA CDDT in clinical microbiology laboratories.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04