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 ORIGINAL ARTICLE
Year : 2010  |  Volume : 28  |  Issue : 3  |  Page : 233-237

Comparison of Helicobacter pylori and Escherichia coli in induction of TNF-α mRNA from human peripheral blood mononuclear cells


1 Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, I.R, Iran
2 Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R, Iran

Correspondence Address:
H Zarkesh-Esfahani
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, I.R
Iran
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Source of Support: The office of Graduate Studies of University of Isfahan, Conflict of Interest: None


DOI: 10.4103/0255-0857.66482

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Purpose: To investigate the difference between the abilities of Helicobacter pylori and Escherichia coli to induce expression of TNF-α in human peripheral blood mononuclear cells (PBMC). Materials and Methods: H pylori was isolated from gastric biopsy specimens. The mononuclear cells were isolated from human blood, cultured, and treated with either intact or sonicated E coli or H pylori, and mRNA expression for TNF-α was detected using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: TNF-α mRNA expression levels were significantly higher in PBMCs stimulated with E coli compared to those stimulated with H pylori at the same number and identical conditions (P < .001). The results also suggest that sonicated bacteria were significantly (P < .001) less stimulatory for PBMCs than intact bacteria for both E coli and H pylori. Conclusions: The ability of different H pylori strains isolated from biopsy samples to stimulate TNF-α from PBMCs was significantly lower than that of E coli. Sonicated bacteria, as compared to intact bacteria, was a very poor inducer of TNF-α mRNA expression, suggesting that the conformation of lipopolysaccharides (LPS) on the outer leaflet of the outer membrane is not totally conserved in sonicated bacteria.






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2004 - Indian Journal of Medical Microbiology
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