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 ORIGINAL ARTICLE
Year : 2010  |  Volume : 28  |  Issue : 3  |  Page : 196-200

Development of polyclonal antisera to clone enterovirus 71 cellular receptor


Department of Pathobiology, Room 304, Faculty of Veterinary Medicine, University of Tabriz, Tabriz - 5176614775, Fellow of Research Centre of Infectious Diseases & Tropical Medicine, Sina Gerenal Hospital, Tabriz, Iran

Correspondence Address:
A B Sioofy-Khojine
Department of Pathobiology, Room 304, Faculty of Veterinary Medicine, University of Tabriz, Tabriz - 5176614775, Fellow of Research Centre of Infectious Diseases & Tropical Medicine, Sina Gerenal Hospital, Tabriz
Iran
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Source of Support: Ministry of Science, Research and Technology of Iran through The University of Tabriz, Iran., Conflict of Interest: None


DOI: 10.4103/0255-0857.66469

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Purpose: Enterovirus 71 (ENV71) is a member of Picornaviridae family and was shown to be of public health concern in the Far East because of the notorious outbreaks it caused, with novel clinical features in the affected patients. In this study we assessed the use of virus capsid protein VP1 in viral receptor research. Material and Methods: The capsid protein (VP1) was cloned, expressed in a prokaryotic system, and purified for immunisation of rabbits. The immunisation was carried out according to the UK Home Office regulations. The polyclonal antisera were collected and tested for reactivity against recombinant and native VP1 of ENV71. Results: Both antisera were reactive against native and partially/fully denatured viral particles. Conclusion: The antisera are functional in receptor studies.






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