Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 1196 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed10501    
    Printed407    
    Emailed15    
    PDF Downloaded511    
    Comments [Add]    
    Cited by others 11    

Recommend this journal

 

 ORIGINAL ARTICLE
Year : 2010  |  Volume : 28  |  Issue : 2  |  Page : 147-151

Identification of Candida species using PCR-RFLP in cancer patients in Iran


1 Department of Medical Mycology and Parasitology , Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Biochemistry and Genetics, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran

Correspondence Address:
T Shokohi
Department of Medical Mycology and Parasitology , Sari Medical School, Mazandaran University of Medical Sciences, Sari
Iran
Login to access the Email id

Source of Support: Mazandaran University of Medical Sciences,, Conflict of Interest: None


DOI: 10.4103/0255-0857.62493

Rights and Permissions

Opportunistic infections caused by Non-Candida albicans. have been increasing. Traditional methods that are used to identify clinical isolates of Candida species are time-consuming and not appropriate for rapid, accurate and reliable identification. Purpose: To identify Candida spp isolated from cancer patients using PCR-restriction enzyme. Materials and ethods: Using universal primers, ITS1 and ITS4, in this study, we could amplify ITS1-5.8S-ITS2 rDNA regions at both 80 clinical isolates and 3 standard strains. The PCR products were digested with two restriction enzymes MspI and BlnI separately. Result: We successfully identified all isolated species using two restriction enzymes (MspI, BlnI). Candida albicans was the most common species (77.5%), followed by C. glabrata (15%), C. tropicalis (5%), C. krusei (2.5%). Although the primers and enzyme had the ability to identify C. parapsilosis, C. guilliermondii, C. dubliniensis, present isolates did not include these among identified ones. Conclusion: RFLP-PCR using ITSI and ITS4 primers and restriction enzyme is a rapid, easy, reliable and also applicable method in clinical laboratory for identification of medically important Candida spp.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04