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CORRESPONDENCE
Year : 2010  |  Volume : 28  |  Issue : 1  |  Page : 80-81
 

Emergence of optochin resistance among S. pneumoniae strains colonizing healthy children in Abidjan


1 Department of Bacteriology, Pasteur Institute of Cote d'Ivoire, Virology-01 BP 490; Department of Microbiology, Unit of Formation and Research of Medical Sciences Abidjan, University of Cocody 01 BPV 166; University Hospital Center of Cocody, Clinical Bacteriology Unit - 01 BP V 33 Abidjan 01, Cote d'Ivoire
2 Department of Bacteriology, Pasteur Institute of Cote d'Ivoire, Virology-01 BP 490, Cote d'Ivoire
3 Department of Microbiology, Unit of Formation and Research of Medical Sciences, Abidjan; University of Cocody 01 BP V 166, University Hospital Center of Cocody, Clinical Bacteriology Unit - 01 BP V 33 Abidjan 01, Cote d'Ivoire

Date of Submission14-Apr-2009
Date of Acceptance28-Jun-2009
Date of Web Publication6-Jan-2010

Correspondence Address:
A Kacou-N'douba
Department of Bacteriology, Pasteur Institute of Cote d'Ivoire, Virology-01 BP 490; Department of Microbiology, Unit of Formation and Research of Medical Sciences Abidjan, University of Cocody 01 BPV 166; University Hospital Center of Cocody, Clinical Bacteriology Unit - 01 BP V 33 Abidjan 01, Cote d'Ivoire

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.58742

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How to cite this article:
Kacou-N'douba A, Okpo S C, Ekaza E, Pakora A, Koffi S, Dosso M. Emergence of optochin resistance among S. pneumoniae strains colonizing healthy children in Abidjan. Indian J Med Microbiol 2010;28:80-1

How to cite this URL:
Kacou-N'douba A, Okpo S C, Ekaza E, Pakora A, Koffi S, Dosso M. Emergence of optochin resistance among S. pneumoniae strains colonizing healthy children in Abidjan. Indian J Med Microbiol [serial online] 2010 [cited 2019 Nov 11];28:80-1. Available from: http://www.ijmm.org/text.asp?2010/28/1/80/58742


Dear Editor,

Streptococcus pneumoniae is one of the most important infection pathogens in the world, particularly in children below five years. This bacteria nasopharyngeal carriage is an important risk factor in the occurrence of pneumococcal diseases. [1] Identification of S. pneumoniae has conventionally been based on phenotypic characteristics. However, optochin-resistant pneumococcal isolates have been reported in the world. This study aims at determining the prevalence of optochin-resistant pneumococcal isolates in children carriers in Abidjan.

A total of 266 strains were isolated in 966 under-five healthy children. Both Isolation and identification of the infection pathogens were based on selective growth of gentamicin blood agar plates, colonies morphological aspect, optochin sensibility, bile solubility, agglutination test and spotting of pneumolysin gene. [2] The optochin sensibility test was performed using six mm diameter optochin disc containing five μg (oxoid, Hampshire, England) within an atmosphere of five per cent CO 2 . Isolates were considered resistant when the inhibition diameter was under 14 mm. Antimicrobial susceptibility was realised using Kirby-Bauer disk diffusion method according CAS-FM 2006 guidelines. When the oxacilin (5 μg) inhibition diameter was less than 26 mm, determination of penicillin minimum inhibitory concentration (MICs) was done using E-test (AB Biodisk, Solna, Sweden) according to CLSI 2009 guidelines. S. pneumoniae (ATCC 49619) was used as control strain. Serotyping was performed using Quellum reaction with antisera produced by the Statens Serum Institute (Copenhagen, Denmark). [3]

Three (1.1%) of the 266 isolates of S. pneumoniae from carriers were found to be optochin-resistant. These strains belonged to three different serotypes - 18, 19 and 23 [Table 1]. They were also multidrug-resistant.

This optochin resistance may be due to mutations. [4] There may also be an association between the use of antimalarial drugs and evolution of optochin resistance. [5] Surveillance of optochin-resistant isolates should be continued to study different mutation types.

 
 ~ References Top

1.Lo WT, Wang CC, Yu CM, Chu ML. Rate of nasopharyngeal carriage, antimicrobial resistance and serotype of Streptococcus pneumoniae among children in Northen Taiwan. J Microbiol Immunol Infect 2003;36:175-81.  Back to cited text no. 1      
2.Nunes S, Sα-Leγo R, de Lencastre H. Optochin resistance among Streptococcus pneumoniae isolates colonizing healthy children in Portugal. J Clin Microbiol 2008;46:321-4.  Back to cited text no. 2      
3.Sψrensen UB. Typing of pneumococci by using 12 pooled antisera. J Clin Microbiol 1993 ;31:2097-100.  Back to cited text no. 3      
4.Verhelst R, Kaijalainen T, De Baere T, Verschraegen G, Claeys G, Van Simaey L, et al. Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolates. J Clin Microbiol 2003;41:3521-5.  Back to cited text no. 4      
5.Pikis A, Campos JM, Rodriguez WJ, Keith JM. Optochin resistance in Streptococcus pneumoniae: Mechanism, significance, and clinical implications. J Infect Dis 2001;184: 582-90.  Back to cited text no. 5      



 
 
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