|Year : 2009 | Volume
| Issue : 4 | Page : 375-376
Vancomycin resistant enterococci in a tertiary care hospital in Mumbai
A De, A Bindlish, S Kumar, M Mathur
Department of Microbiology, L.T.M. Medical College, Sion, Mumbai - 400 022, India
|Date of Submission||04-Dec-2008|
|Date of Acceptance||16-May-2009|
|Date of Web Publication||4-Sep-2009|
Department of Microbiology, L.T.M. Medical College, Sion, Mumbai - 400 022
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
De A, Bindlish A, Kumar S, Mathur M. Vancomycin resistant enterococci in a tertiary care hospital in Mumbai. Indian J Med Microbiol 2009;27:375-6
|How to cite this URL:|
De A, Bindlish A, Kumar S, Mathur M. Vancomycin resistant enterococci in a tertiary care hospital in Mumbai. Indian J Med Microbiol [serial online] 2009 [cited 2020 Mar 31];27:375-6. Available from: http://www.ijmm.org/text.asp?2009/27/4/375/55451
The increasing occurrence of Enterococcus species , worldwide, since late 1980s, is of particular concern due to the emergence of Vancomycin Resistant Enterococci (VRE).  VRE has also been reported from some parts of India. , The appearance of VRE has limited the therapeutic options available for clinicians.
A study was undertaken in this hospital to detect vancomycin resistance in enterococcal isolates using three methods and compare the three methods. The methods included - KBDDM, Vancomycin agar screen method and MIC detection by macrobroth dilution method. A total of 200 enterococcal isolates - 65 from urine, 58 from blood, 22 from Foley's catheter tips, 21 from wound swabs, 18 from pus and 16 from fluids (ascetic fluid 12, cerebrospinal fluid 2 and peritoneal dialysis fluid 2) were included in the study. They were identified and speciated by standard biochemical tests. 
Susceptibility to vancomycin was performed by Kirby-Bauer Disc Diffusion Method (KBDDM)  on Mueller Hinton Agar by using 30µg vancomycin disc (HiMedia). Vancomycin resistance was also determined by Vancomycin agar screen method using 6µg/ml of vancomycin incorporated in Brain Heart Infusion (BHI) agar. Minimum Inhibitory Concentration (MIC) of all the isolates were done by Macrobroth dilution method, using dilutions of vancomycin ranging from 2 µg/ml to 512 µg/ml. Susceptibility to teicoplanin was also done by KBDDM, in isolates showing MIC ≥ 4 µg/ml. 
Out of the 200 Enterococcus species , 55% (110) were Enterococcus fecium , 31% (62) were Enterococcus fecalis and 14% (28) were other Enterococcus species.
Two isolates (1%) were resistant to vancomycin by KBDDM. By Vancomycin agar screen method, three isolates showed growth, giving an overall VRE positivity of 1.5%. The vancomycin MIC for these three isolates were 8 µg/ml for one and 128 µg/ml for two, while the remaining isolates had MIC less than or equal to 4µg/ml.
The three enterococcal isolates having MIC greater than 4 µg/ml were from two patients - one each from Foley's catheter tip and ascitic fluid of the same patient and the third from Foley's catheter tip of another patient. The former patient was a 11-year-old female child diagnosed to have right sided empyema with acute renal failure, with splenomegaly and mild ascites. She developed facial and pedal oedema later. She was given repeated blood transfusion and peritoneal dialysis was also done. She was started on amikacin and cefotaxime at the time of admission and later on linezolid, as the isolates showed in-vitro susceptibility to linezolid. She responded to the treatment and was subsequently discharged. The other patient was a 64-year-old male having diabetes mellitus with chronic renal failure. He also responded to linezolid and was discharged. Both the patients were lost to follow-up. All the three VRE isolates were E. fecium and resistant to teicoplanin.
According to CLSI guidelines,  MIC of vancomycin for enterococci between 8-16 µg/ml is considered as intermediate resistant and MIC greater than or equal to 32µg/ml is considered resistant. Therefore in this study, 0.5% showed intermediate resistance and one per cent were resistant to vancomycin by MIC.
In this study, MIC of vancomycin was between 8µg/ml-128µg/ml in 3 (1.5%) enterococcal isolates - two from Foley's catheter tips and one from ascitic fluid. Taneja et al. reported eight enterococcal isolates having MIC ranging from 8-32µg/ml by agar dilution method.  Five of them developed nosocomial urinary tract infection (UTI), 2 had community acquired UTI and 1 had asymptomatic bacteriuria. In their study also, E. fecium was the commonest amongst VRE, as in this study.
In this study, all the three VRE isolates were E. fecium and were resistant to teicoplanin, so they were of VanA phenotype. Mathur et al .  have also reported 80% of VanA phenotype. Though one study have reported 25% mortality among VRE isolates,  no mortality was reported in this study.
Two urine samples were also received from the female child while on antibiotics. Both did not show any growth. So isolate from Foley's catheter tip in this patient can be a probable colonizer. As no VRE was isolated from other patients during the same period, these cases may be community acquired. Report from developed countries suggest that VRE colonization can frequently occur in community.  An Indian study has also reported community-acquired VRE.  VRE exists elsewhere in the environment including animal faeces and foods of animal origin. Therefore transmission of VRE from these sources results in an increased human reservoir of VRE colonization.  Control of these cases therefore requires community-based initiative.
The Vancomycin agar screen method, which is used for detection of presumptive resistance, correlated well with Macrobroth dilution method in this study. Comparing KBDDM with Macrobroth dilution method by Chi-square test, the results were not significant (' P ' greater than 0.005).
This study signals the emergence of VRE in this hospital and also highlights the importance of screening for VRE in enterococci isolated from various samples. As MIC detection is a laborious procedure, all enterococcal isolates can be screened by Vancomycin agar screen method and only those isolates positive by this method can be tested further for vancomycin MIC, as both the above methods correlated well in this study.
All laboratories should have effective detection methods for vancomycin resistance, which will be helpful in reducing the morbidity and mortality due to VRE in hospitalized patients. VRE with community acquired sources should be detected early as this will limit the spread of VRE to the hospital environment. Surveillance of family members of recently discharged patients known to be colonized or VRE-infected should be done. Surveillance of the community to detect reservoirs of VRE should also be done from time to time. 
| ~ References|| |
|1.||Zhanel GG, Laing NM, Nichol KA, Palatnick LP, Noreddin A, Hisanaga T, et al . Antibiotic activity against urinary tract infection (UTI) isolates of vancomycin resistant enterococci: Results from the 2002 North American Vancomycin Resistant Enterococci susceptibility study (NAVRESS). J Antimicrob Chemother 2003;52:382-8. |
|2.|| Mathur P, Kapil A, Chandra R, Sharma P, Das B. Antimicrobial resistance in Enterococcus fecalis at a tertiary care center of Northern India. Indian J Med Res 2003;118:25-8. |
|3.||Taneja N, Rani P, Emmanuel R, Sharma M. Significance of vancomycin resistant enterococci from urinary specimens at a tertiary care center in Northern India. Indian J Med Res 2004;119:72-4. |
|4.||Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC Jr. The gram positive cocci. Part II: Streptococci, enterococci and the Streptococcus -like bacteria. In Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 5 th Ed. Lippincott, Philadelphia. 1997; pp577-650. |
|5.||Performance Standards for Antimicrobial Susceptibility Testing. Seventeenth Informational Supplement. Clinical Laboratory Standards Institute. 2007; M100-S17;27(1):p53. |
|6.||McDonald LC, Kuchnert MJ, Tenover FC, Jarvis WR. Vancomycin resistant enterococci outside the health care setting: Prevalence, sources and public health implications. Emerg Infect Dis 1997;3:311-7. |
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