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Year : 2009  |  Volume : 27  |  Issue : 2  |  Page : 149-152

Detection of porcine rotavirus from tissue and faecal specimens


Department of Microbiology, International Centre for Cardio Thoracic and Vascular Diseases, Frontier Lifeline Pvt Ltd, Dr. K.M. Cherian Heart Foundation, R-30-C, Ambattur Industrial Estate Road, Chennai-600 101, India

Correspondence Address:
Suji Prabha
Department of Microbiology, International Centre for Cardio Thoracic and Vascular Diseases, Frontier Lifeline Pvt Ltd, Dr. K.M. Cherian Heart Foundation, R-30-C, Ambattur Industrial Estate Road, Chennai-600 101
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.49430

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Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute diarrhoea. The purpose of this study was to evaluate the porcine intestinal tissue scaffold for Rotavirus and to study the incidence of this virus among pig herds. Only one isolate was successfully adapted to grow in cell line MA 104 from faecal samples. This isolate was further confirmed by reverse transcriptase polymerase chain reaction and sequence analysis.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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