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 ORIGINAL ARTICLE
Year : 2009  |  Volume : 27  |  Issue : 1  |  Page : 30-34

In vitro inhibition of Helicobacter pylori urease with non and semi fermented Camellia sinensis


1 Microbiology Department, Science and Research Branch of IAU, Tehran, Iran
2 Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3 Microbiology Department, Iran Medical School, Tehran, Iran
4 Basic Sciences Department, Lahijan Azad University, Guilan, Iran

Correspondence Address:
A Ghaemi
Faculty of Medicine, Golestan University of Medical Sciences, Gorgan
Iran
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Source of Support: None, Conflict of Interest: None


PMID: 19172056

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Purpose: Helicobacter pylori is the etiological agent in duodenal and peptic ulcers. The growing problem of antibiotic resistance by the organism demands the search for novel compounds, especially from natural sources. This study was conducted to evaluate the effect of Camellia sinensis extracts on the urease enzyme that is a major colonization factor for H. pylori. Methods: Minimum inhibitory concentrations of nonfermented and semifermented C. sinensis methanol: water extracts were assessed by broth dilution method. Examination of the urease function was performed by Mc Laren method, and urease production was detected on 12% SDS polyacrylamide gel electrophoresis from whole cell and membrane bound proteins. Results: Both extracts had inhibitory effects against H. pylori and urease production. At a concentration of 2.5 mg/ml of nonfermented extract and 3.5 mg/ml of semifermented extract the production of Ure A and Ure B subunits of the urease enzyme were inhibited completely. A concentration of 4 mg/ml of nonfermented and 5.5 mg/ml of semifermented extract were bactericidal for H. pylori. Conclusions: C. sinensis extracts, especially the nonfermented, could reduce H. pylori population and inhibit urease production at lower concentrations. The superior effect of nonfermented extract is due to its rich polyphenolic compounds and catechin contents.






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2004 - Indian Journal of Medical Microbiology
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