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Year : 2008  |  Volume : 26  |  Issue : 4  |  Page : 398

Imipenem resistant metallo β lactamse producing Pseudomonas aeruginosa

Department of Microbiology, Dr. L.H. Hiranandani Hospital, Powai, Mumbai - 400 076, Maharashtra, India

Date of Submission09-Jan-2008
Date of Acceptance01-Feb-2008

Correspondence Address:
A Varaiya
Department of Microbiology, Dr. L.H. Hiranandani Hospital, Powai, Mumbai - 400 076, Maharashtra
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.43565

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How to cite this article:
Chacko B, Varaiya A, Dedhia B. Imipenem resistant metallo β lactamse producing Pseudomonas aeruginosa. Indian J Med Microbiol 2008;26:398

How to cite this URL:
Chacko B, Varaiya A, Dedhia B. Imipenem resistant metallo β lactamse producing Pseudomonas aeruginosa. Indian J Med Microbiol [serial online] 2008 [cited 2020 Jun 4];26:398. Available from:

Dear Editor,

Pseudomonas aeruginosa, an important nosocomial pathogen is difficult to treat because of high antimicrobial resistance. [1] The common form of resistance is mediated by lack of drug penetration and / or carbapenem hydrolyzing β-lactamases. [2] Detection of metallo β lactamase (MBL)-producing P. aeruginosa is crucial for the optimal treatment of patients. The present study was undertaken to study Imipenem resistant P. aeruginosa for MBL production.

Seventy six Pseudomonas aeruginosa isolates were obtained from various types of specimens (38 respiratory secretions, 10 body fluids, 10 pus swabs, 8 catheter tips, 5 catheterized urine samples and 5 - blood culture) over a one year period from October 2006 to September 2007. Isolates were identified by standard laboratory techniques and antibiotic sensitivity was performed on Mueller Hinton Agar plates by Kirby Bauer disc diffusion method using commercially available discs (Hi Media) according to CLSI guidelines. [3] Disc approximation test was used to detect MBL production - the zone size difference between imipenem alone and imipenem+EDTA being ≥ 7 mm. P. aeruginosa ATCC 27853 was used as a negative control.

In this study 11 out of 76 (14.47%) strains were resistant to imipenem. Of these 11 strains, 8 (10.53%) were found positive for MBL production. Strains producing metallo beta lactamase were 100% sensitive to colistin, 81% to piperacillin/ tazobactam and 54% to gatifloxacin. Thus, MBL production was observed in multiresistant P. aeruginosa which were imipenem resistant.

EDTA impregnated imipenem discs can be easily used and are inexpensive for primary screening for multi drug resistant P. aeruginosa isolates. Sensitivity to Colistin represents the best treatment option for metallo betalactamase producing P. aeruginosa .

 ~ References Top

1.Poirel L, Nordmann P. Acquired carbapenem-hydrolyzing beta-lactamases and their genetic support. Curr Pharma Biotech 2002;3:117-27.  Back to cited text no. 1    
2.Hemalatha V, Sekar U, Vijaylakshmi K. Detection of Metallo betalactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J Med Res 2005;122:148-52.  Back to cited text no. 2    
3.Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Susceptibility Tests; 9 th ed. Vol.26 2006. p. M2-A9.  Back to cited text no. 3    

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