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CORRESPONDENCE
Year : 2008  |  Volume : 26  |  Issue : 2  |  Page : 197-198
 

Bacteriological and molecular studies of group A streptococcal pharyngitis in a south Indian hospital


1 Department of Microbiology, Government Stanley Medical College, Chennai - 600 001, India
2 Department of Clinical Microbiology, Christian Medical College, Vellore - 632 004, India

Date of Submission19-Aug-2007
Date of Acceptance06-Sep-2007

Correspondence Address:
C Sindhulina
Department of Microbiology, Government Stanley Medical College, Chennai - 600 001
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.40545

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How to cite this article:
Sindhulina C, Geethalakshmi S, Thenmozhivalli P R, Jose J M, Brahmadathan K N. Bacteriological and molecular studies of group A streptococcal pharyngitis in a south Indian hospital. Indian J Med Microbiol 2008;26:197-8

How to cite this URL:
Sindhulina C, Geethalakshmi S, Thenmozhivalli P R, Jose J M, Brahmadathan K N. Bacteriological and molecular studies of group A streptococcal pharyngitis in a south Indian hospital. Indian J Med Microbiol [serial online] 2008 [cited 2019 Nov 17];26:197-8. Available from: http://www.ijmm.org/text.asp?2008/26/2/197/40545


Dear editor,

A high level of heterogeneity among the strains of group A streptococci (GAS) circulating in India has been noted. [1],[2],[3] This study was undertaken to determine the prevalence of GAS pharyngitis in children and identify the emm types of GAS associated with such infections. Two hundred school children aged four to thirteen years with complaints of sore throat seen at the Pediatric outpatient clinics of a tertiary care hospital were included in the study. The beta-haemolytic streptococci isolated from throat swabs from children with pharyngitis were identified by serogrouping (co-agglutination method) and then subjected to emm typing by an automated gene sequencing technique. [4] As defined by the isolation of group A streptococci, the prevalence of GAS pharyngitis was found to be 6% (12 out of 200). This pilot study shows that symptoms such as pain on swallowing and absence of coryza may be used as possible indicators of GAS pharyngitis [Table - 1]. Ten different emm types were found among the 11 isolates. They were emm 1-2.2, emm 55, emm 57, emm 71 (2 isolates), emm 74, emm 82.1, emm 95, emm 112.2, st 11014 and a unique sequence, designated as subtype number st2002.1. [5] Our results on emm typing show distinct differences from those reported earlier from Chennai. [2] Our finding on the high level of heterogeneity among GAS isolates causing pharyngitis shows that the molecular epidemiology of streptococcal pharyngitis is distinctly different in endemic areas. However, more such hospital-based studies on GAS isolated from acute and invasive GAS infections are necessary to confirm this observation.


 ~ Acknowledgement Top


We are grateful to Dr. S. Savithri, MD, Deputy Director, Clinical and Diagnostic Section, King Institute of Preventive Medicine, Guindy, Chennai, for providing the facilities and support in preparing the group A co agglutination reagent as a project.

 
 ~ References Top

1.Brahmadathan KN, Gladstone P. Microbiological diagnosis of streptococcal pharyngitis: Lacunae and their implications. Indian J Med Microbiol 2006;26:92-6.  Back to cited text no. 1    
2.Menon T, Whatmore AM, Srivani S, Kumar MP, Anbumani N, Rajaji S. Emm types of Streptococcus pyogenes in Chennai. Indian J Med Microbiol 2001;19:161-2.  Back to cited text no. 2    
3.Sagar V, Bakshi DK, Nandi S, Ganguly NK, Kumar R, Chakraborti A. Molecular heterogeneity among north Indian isolates of Group A streptococcus. Lett Appl Microbiol 2004;39:84-8.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Beall B, Facklam R, Thompson T. Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci. J Clin Microbiol 1996;34:953-8.  Back to cited text no. 4  [PUBMED]  [FULLTEXT]
5.Subtype number st2002. 1 (CDC M protein typing ftp database) Available from: ftp://ftp.cdc.gov/pub/infectious_diseases/biotech/emmsequ/st2002.1. [Last accessed on 2008 April 13].  Back to cited text no. 5    



 
 
    Tables

  [Table - 1]

This article has been cited by
1 Prevalence of emm types of Group A streptococci recovered from school children and hospital patients in Bangalore City, India
K. Lakshmana Gowda, J. John Melbin, S. A. Patil, S. R. Bindu Rani, M. K. Sanjay, C. T. Shivannavar, K. N. Brahmadathan
World Journal of Microbiology and Biotechnology. 2011; 27(2): 319
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