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 ORIGINAL ARTICLE
Year : 2008  |  Volume : 26  |  Issue : 2  |  Page : 127-131

Advantage of using a home-made elisa kit for detection of Helicobacter pylori infection over commercially imported kits


1 Biotechnology Research Center, Pasteur Institute of Iran, Iran
2 Department of Immunology, Pasteur Institute of Iran, Iran
3 Digestive Diseases Research Center, Tehran University of Medical Science, Shariati Hospital, Tehran, Iran

Correspondence Address:
M Mohammadi
Biotechnology Research Center, Pasteur Institute of Iran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.40525

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Purpose: To evaluate a home-made ELISA kit for detection of Helicobacter pylori (Hp) infection and comparison of its immunologic criteria with those of foreign commercial kits. Methods: A home-made IgG ELISA kit was developed using soluble antigenic fractions of Hp proteins. Confirmed sera were tested and serological criteria were evaluated through assessment of 199 serum samples. Results: The accuracy, sensitivity and specificity values of home-made kit were 92, 92 and 90.4%, respectively. These immunologic criteria for Trinity kit were 95.2, 95.2 and 95% in comparison with IBL kit (91.3, 92.2 and 88.5%), BIOHIT kit (72.4, 41.6 and 94.1%) and HelicoBlot2.1 (94.2, 93.4 and 100%). Kappa agreement assessment demonstrated that two of the imported ELISA kits had fair to moderate agreement with the home-made kit while the other one had a poor agreement value. Conclusions: Apart from comparable values between the home-made kit and the most efficient imported kit (Trinity) there was significant cost benefit. Therefore, we recommend the home-made kit as a suitable substitution for detection of Hp infection in the Iranian population.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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