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BRIEF COMMUNICATION
Year : 2008  |  Volume : 26  |  Issue : 1  |  Page : 62-64
 

Antimicrobial susceptibility profile of Neisseria gonorrhoeae at STI clinic


1 Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, New Delhi - 110 095, India
2 Department of Dermatology and STD, UCMS and GTB Hospital, Dilshad Garden, New Delhi - 110 095, India

Date of Submission26-Jun-2006
Date of Acceptance26-Mar-2007

Correspondence Address:
C Shilpee
Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, New Delhi - 110 095
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.38861

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 ~ Abstract 

A total of 100 consecutive patients who attended a sexually transmitted infections clinic were studied. Thirteen had gonococcal urethritis, of which 10 showed growth of Neisseria gonorrhoeae on culture. All the isolates were tested for antimicrobial susceptibility by Australian Gonococcal Surveillance Programme (AGSP) method and beta lactamase production by chromogenic cephalosporin test. Four patients were co-infected with each of the following: HIV, HBV and Chlamydia trachomatis . Gonococcal urethritis (13%) was found more in male patients. Ten percent gonococcal isolates were penicillinase-producing N. gonorrhoeae , and another 10% were tetracycline-resistant N. gonorrhoeae .


Keywords: Neisseria gonorrhoeae, penicillin resistant, tetracycline resistant


How to cite this article:
Shilpee C, Ramachandran V G, Das S, Bhattacharya S N. Antimicrobial susceptibility profile of Neisseria gonorrhoeae at STI clinic. Indian J Med Microbiol 2008;26:62-4

How to cite this URL:
Shilpee C, Ramachandran V G, Das S, Bhattacharya S N. Antimicrobial susceptibility profile of Neisseria gonorrhoeae at STI clinic. Indian J Med Microbiol [serial online] 2008 [cited 2019 Jul 24];26:62-4. Available from: http://www.ijmm.org/text.asp?2008/26/1/62/38861


Gonorrhoea, a disease well documented from ancient times, continues to defy man's attempt to control it. Gonococcal infections and their complications are amongst the most frequent communicable diseases in many countries. [1] Gonococci have been adept at developing resistance to several commonly used antimicrobials. The failure to cure a case of gonorrhoea has public health implications due to the potential for continued transmission and rapid emergence of antimicrobial resistance. Moreover, a number of sexually transmitted infections (STIs) have been identified as facilitating the spread of HIV. Presently, more than 5.26 million people in India are HIV seropositive. The present study was done to isolate  Neisseria More Details gonorrhoeae from the patients attending STI clinic at a large tertiary care hospital in East Delhi, which caters to the semi-urban and migratory population. Antimicrobial susceptibility was assessed to determine the sensitivity pattern of these isolates. The correlation of N. gonorrhoeae with other STIs, including HIV and their influence on phenotypic behaviour of N. gonorrhoeae , has also been studied.


 ~ Materials and Methods Top


Hundred consecutive patients who attended the STI clinic at Guru Teg Bahadur Hospital, Delhi, were included as subjects. The subjects reported one or more of the complaints as enunciated by WHO in the syndromic approach to the diagnosis of STIs. Detailed history, demographical and clinical features were recorded. Urethral and endocervical swabs were collected from males and females, respectively, and subjected to direct examination of Gram staining and culture plate inoculation. A presumptive diagnosis of gonococcal infection was made on observing polymorphonuclear leucocytes (PMNLs) with gram-negative intracellular diplococci (ICDC). If the smear showed four or more PMNLs in the absence of gram-negative ICDC, a presumptive diagnosis of non-gonococcal urethritis (NGU) was made. [2] For the isolation of N. gonorrhoeae , the swabs were directly inoculated on chocolate agar plate containing vancomycin, colistin and amphotericin-B and incubated in 5-10% carbon dioxide for 24-48 hours. The isolates were identified as N. gonorrhoeae on the basis of colony morphology, Gram staining, oxidase test and rapid carbohydrate utilization test (RCUT). [3]

Antimicrobial susceptibility testing of N. gonorrhoeae was done by disc diffusion technique following the AGSP method. [3] The antimicrobial discs used were penicillin (0.5 IU), nalidixic acid (30 μg), ciprofloxacin (1 μg), ceftriaxone (0.5 μg), spectinomycin (100 μg) and tetracycline (10 μg). All the isolates were also tested for beta lactamase production by chromogenic cephalosporin method. [3]


 ~ Results Top


A total of 13 patients were found to have gonococcal infection of which 11 were males and 2 females. The ages varied between 15 and 40 years, and a majority were in the age group of 25-30 years. There was a history of promiscuity in all male patients and one female patient. Patients with gonorrhoea (10/13) mainly presented with profuse, thick, yellow discharge. In seven patients, N. gonorrhoeae could be detected both by microscopy and culture, whereas three patients were detected positive by microscopy only and rest by culture alone.

In our study, 20% gonococcal isolates were penicillin resistant and 10% produced penicillinase [Table - 1]. The trends of antimicrobial susceptibility pattern of N. gonorrhoeae as documented in various studies carried out in India are depicted in [Table - 2].

Concomitant infection was seen in four male patients having gonococcal urethritis. One was seropositive for HIV; two for HBsAg, and in one patient, chlamydial antigen was detected.


 ~ Discussion Top


In our study, gonococcal urethritis (13%) was found more in male patients in the age group of 25-30 years with promiscuous behaviour and predominantly presented with profuse, thick, yellow discharge. The importance of Gram stained smear in the diagnosis of gonorrhoea was also well highlighted, with a sensitivity of 77% (10/13). Acquisition of multiple STIs is a widespread problem and has been attributed to behavioural risk factors. Thirty percent (4/13) of patients with gonococcal infections in the present study were found to be co-infected with HIV, Chlamydia and HBV. All these patients had recurrent episodes of gonococcal infections, although clinical manifestation was same as that of patients with gonococcal infection alone. Such recurrence of gonococcal infection in patients with multiple STIs highlights the vulnerability of such population in acquiring STIs and eventually becoming reservoir of drug-resistant organisms.

Our observation also reinforces the emergence of penicillin, quinolone and tetracycline resistance in N. gonorrhoeae isolates. In our study population, 10% (1/10) gonococcal isolates were penicillinase-producing N. gonorrhoeae (PPNG) as compared to 8-17.8% reported from other parts of India. Ten percent (1/10) isolates were found to be resistant to both ciprofloxacin and nalidixic acid, and another 10% (1/10) were tetracycline-resistant N. gonorrhoeae (TRNG). All strains were sensitive to spectinomycin and ceftriaxone. PPNG isolates from Asia are proline auxotrophs and carry a 4.4 MDa plasmid. [10] There is an additional conjugative 24.5 MDa large plasmid associated with it. The presence of the associated conjugative plasmid in Asian strain is responsible for rapid spread of resistance to other gonococci. Tetracycline resistance in gonococci may be mediated by chromosomal or plasmid determinants. The location of the tet M gene on the transferable plasmid has perhaps served to enhance the transmission efficiency of tetracycline resistance in N. gonorrhoeae strains, causing the rapid spread of TRNG. In our study, the percentage resistance of N. gonorrhoeae isolates to the panel of antibiotics (Pn, TC, Cip, NA) differs from isolates of the same organism from other part of Delhi [Table - 2], suggesting the existence of different clones of gonococcal strain circulating in and around Delhi. The failure to cure a case of gonorrhoea has public health implications due to its potential for continued transmission and rapid emergence of antimicrobial resistance. It is said that regimens for the treatment of gonorrhoea should have efficacies that approach 100%, and treatment with efficacies less than 95% should never be used. [11] Hence, continuous surveillance of antibiotic resistance pattern is necessary for guiding therapy in high-risk population. Moreover, the prevalent trend of gonococcal infection suggests the existence of heterogeneous population depending upon the local pattern of their high-risk behaviour. Therefore, reduction in the severity of the disease will be possible by adopting preventive measures and continuous education of safer sexual behaviour through health care authorities.

 
 ~ References Top

1.World Health Organization (Geneva). Neisseria gonorrhoeae and gonococcal infections. TRS 616. WHO: Geneva; 1978.  Back to cited text no. 1    
2.Bowie WR. Comparison of gram staining and first voided urine sediment in the diagnosis of urethritis. Sex Transm Dis 1978;5:39-42.  Back to cited text no. 2  [PUBMED]  
3.Laboratory diagnosis of gonorrhoea. WHO regional publication: South-East Asia. New Delhi, India; 1999.  Back to cited text no. 3    
4.Jain SK, Kulkarni MG, Banker DD. Antibiotic susceptibility pattern of gonococcal isolates. Indian J Med Sci 1994;48:233-6.  Back to cited text no. 4    
5.Bhalla P, Sethi K, Reddy BS, Mathur MD. Antimicrobial susceptibility and plasmid profile of Neisseria gonorrhoeae in India (New Delhi). Sex Transm Infect 1998;74:210-2.  Back to cited text no. 5  [PUBMED]  [FULLTEXT]
6.Gupta CM, Sanghi S, Sayal SK, Das AL, Prasad GK. Clinical and bacteriological study of urethral discharge. Indian J Dermatol Venereol Leprol 2001;67:185-8.  Back to cited text no. 6    
7.Bhalla P, Vidhani S, Reddy BS, Chowdhry S, Mathur MD. Rising quinolone resistance in Neisseria gonorrhoeae isolates from New Delhi. Indian J Med Res 2002;115:113-7.  Back to cited text no. 7  [PUBMED]  
8.Bala M, Ray K, Gupta SM. Comparison of disc diffusion results with minimum inhibitory concentration (MIC) values for antimicrobial susceptibility testing of Neisseria gonorrhoeae . Indian J Med Res 2005;122:48-51.  Back to cited text no. 8  [PUBMED]  [FULLTEXT]
9.Chowdhry S, Pandhi D, Vidhani S, Bhalla P, Reddy BS. High incidence of treatment failure of Neisseria gonorrhoeae isolates to ciprofloxacin in male gonococci urethritis in Delhi. Int J STD AIDS 2002;13:564-7.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]
10.Sparling PF. Biology of Neisseria gonorrhoeae. In : Holmes KK, Mardh PA, Sparling PF, Lemon SM, Piot P, et al , editors. Sexually transmitted diseases, 3 rd ed. McGraw Hill: New York; 1999. p. 131-48.  Back to cited text no. 10    
11.Agrawal S, Garg VK, Agarwalla A, Joshi A, Ramachandran VG. Nepal J Dermatol Venerol Leprol 2002;2:17-9.  Back to cited text no. 11    



 
 
    Tables

  [Table - 1], [Table - 2]

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