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Year : 2007  |  Volume : 25  |  Issue : 4  |  Page : 391-394

Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent In situ hybridization


1 Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia
2 School of Medicine and Health Sciences, Monash University Malaysia, Jalan PJS 11/5, Bandar Sunway, 46150 Petaling Jaya, Malaysia

Correspondence Address:
S D Sekaran
Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur
Malaysia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.37346

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Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04