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 ORIGINAL ARTICLE
Year : 2007  |  Volume : 25  |  Issue : 4  |  Page : 346-350

Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals


1 Department of Infectious Diseases and Clinical Microbiology, Dicle University Hospital, Diyarbakir, Turkey
2 Kocaeli University Hospital, Kocaeli, Turkey
3 Firat University Hospital, Elazig, Turkey
4 Ondokuzmayis University Hospital, Samsu, Turkey
5 Kocatepe University Hospital, Afyon, Turkey
6 Karadeniz Technical University Hospital, Trabzon, Turkey

Correspondence Address:
S Hosoglu
Department of Infectious Diseases and Clinical Microbiology, Dicle University Hospital, Diyarbakir
Turkey
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.37336

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Purpose: To study the prevalence of TEM-, SHV- and GES-type β -lactamases among Escherichia coli and Klebsiella pneumoniae strains having ceftazidime MICs higher than 2 mg/L. Methods: A total of 63 E. coli and 41 K. pneumoniae isolated from five different university hospitals were studied for the existence of TEM-, SHV- and GES-type β -lactamases. Susceptibility tests were carried out according to the criteria of National Committee for Clinical Laboratory Standards. MICs were obtained by agar dilution method. Existence of extended-spectrum β -lactamases (ESBLs) were assessed by double-disc synergy test (DDST). Existence of the above-mentioned β -lactamase genes were studied both by PCR with specific oligonucleotide primers and isoelectric focusing methods. Results: None of the isolates were carbapenem-resistant. DDSTs were positive in 50 (79.3%) and 33 (80.5%) of E. coli and K. pneumoniae , respectively. TEM gene was detected in 41 (65.1%) and 19 (46.3%), whereas SHV gene in 18 (28.6%) and 20 (48.8%) of E. coli and K. pneumoniae strains, respectively. GES genes were not detected. Conclusions: TEM and SHV genes are highly prevalent among ESBL-producing E. coli and K. pneumoniae , whereas GES-type ESBLs are absent and found not to be responsible of ceftazidime resistance in Turkish hospitals.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04