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Year : 2007  |  Volume : 25  |  Issue : 3  |  Page : 249-252

Determination of hepatitis C virus genotypes by melting-curve analysis of quantitative polymerase chain reaction products


Department of Pathology and Laboratory Medicine - Microbiology Division, Zayed Military Hospital, Abu Dhabi, United Arab Emirates

Correspondence Address:
M S Alfaresi
Department of Pathology and Laboratory Medicine - Microbiology Division, Zayed Military Hospital, Abu Dhabi
United Arab Emirates
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.34768

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Hepatitis C virus (HCV) is the major causative agent of non-A and non-B viral hepatitis. Factors associated with disease progression following HCV infection include the viral genotype, the patient's alcohol consumption, and viral load. In this study, the COBAS AMPLICOR HCV MONITOR test, a commercially available quantitative assay for HCV RNA, was used for HCV genotyping analysis. Amplification products obtained from 100 HCV-positive cases were subjected to real-time polymerase chain reaction (PCR) typing using a single pair of fluorescence resonance energy transfer (FRET) probes and melting-curve analysis. Of 100 samples tested, two inhibited the PCR, two samples yielded discrepancies between our results and the reference laboratory results, and the remaining samples provided correct typing. The present report suggests that HCV genotypes can be determined rapidly with FRET probes directly from COBAS AMPLICOR MONITOR test PCR products.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04