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CORRESPONDENCE
Year : 2007  |  Volume : 25  |  Issue : 2  |  Page : 177-178
 

Circulating phage type of Vibrio cholerae in Mumbai


1 Department of Microbiology, Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra, India
2 Department of Microbiology, BJ Medical College, Sassoon General Hospital, Pune - 411 001, Maharashtra, India
3 Department of Microbiology, Government Medical College, Sassoon General Hospital, Pune - 411 001, Maharashtra, India

Correspondence Address:
S D Turbadkar
Department of Microbiology, Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.32738

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How to cite this article:
Turbadkar S D, Ghadge D P, Patil S, Chowdhary A S, Bharadwaj R. Circulating phage type of Vibrio cholerae in Mumbai. Indian J Med Microbiol 2007;25:177-8

How to cite this URL:
Turbadkar S D, Ghadge D P, Patil S, Chowdhary A S, Bharadwaj R. Circulating phage type of Vibrio cholerae in Mumbai. Indian J Med Microbiol [serial online] 2007 [cited 2018 Nov 17];25:177-8. Available from: http://www.ijmm.org/text.asp?2007/25/2/177/32738


Dear Editor,

Cholera is endemic in several states of India. Bacteriophage typing is widely accepted as a convenient and highly discriminatory method of identifying epidemic strains of Vibrio cholerae . It is important to monitor the prevalent phage types within an area as introduction of new phage type may herald the onset of an outbreak.

A total of 200 faecal samples, received between January and December 2004 from patients with acute diarrhoea, were screened for the presence of V. cholerae by standard laboratory methods, [1] and identified by routine biochemical tests and confirmed by slide agglutination with antisera obtained from National Institute of Cholera and Enteric Diseases, Kolkata. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion method. [2] The strains were then sent for phage typing to National Institute of Cholera and Enteric Diseases, Kolkatta. A total of 41 (20.5%)  V.cholerae Scientific Name Search  were isolated. All the strains were biotype El Tor, serotype Ogawa and belonged to phage type 4. These strains were typable with new scheme and were clustered in to type 27 (97.5%) and type 23 (2.4%) respectively. Vibrio cholerae isolates showed maximum sensitivity in vitro to gentamicin (92.6%) and chloramphenicol (87.8%) respectively [Table - 1].

In spite of the complexities of the environment and a large migratory population in Mumbai, all the isolates belonged to Basu and Mukerjee phage type 4. Type 2 and 4 are the prevalent biotypes in India at present [3] but consistently getting only a single biotype over the year was surprising. However, the new scheme was more discriminatory and could identify two circulating types. Even then the majority belonged to types 27 (97.5 %) and only 2.4 % were type 23. These were found in the month of August and were likely to be imported phage type into the city.

Though prevalence of multi-drug resistant Vibrio cholerae has been reported from parts of India, [4],[5] we did not encounter any such strains. A decrease in sensitivity to ampicillin and tetracycline was observed.



 
 ~ References Top

1.Cruickshank R, Duguid JP, Marimion BP, Swann RH. Medical Microbilogy. Vol. II, 12 th ed. Churchill Livingstone: Edinburgh; 1985. p. 440-2.   Back to cited text no. 1    
2.Bauer AW, Kirby WM, Sherris JC, Turck MC. Antibiotic susceptibility testing by standardized single disc method. Am J Clin Pathol 1966; 45 :493-6 .   Back to cited text no. 2    
3.Sundaram SP, Revathi J, Sarkar BL, Bhattacharya SK. Bacteriological profile of cholera in Tamil Nadu (1980-2001). Indian J Med Res 2002; 116 :258-63.   Back to cited text no. 3  [PUBMED]  
4. Taneja N, Mohan B, Khurana S, Sharma M. Antimicrobial resistance in selected bacterial enteropathogens in north India. Indian J Med Res 2004; 120 :39-43.   Back to cited text no. 4    
5. Mathur M, De A, Saraswathi K, Varaiya A, Athalye S. Vibrionaceae from cases of acute diarrhoea and their antimicrobial sensitivity pattern - A five year prospective study. Indian J Med Microbiol 2003; 21 :199-201.  Back to cited text no. 5    



 
 
    Tables

  [Table - 1]

This article has been cited by
1 Plasmid mediated antibiotic resistance of Vibrio cholerae O1 biotype El Tor serotype Ogawa associated with an outbreak in Kolkata, India
Mandal, S., DebMandal, M., Pal, N.K.
Asian Pacific Journal of Tropical Medicine. 2010; 3(8): 637-641
[Pubmed]
2 Plasmid mediated antibiotic resistance of Vibrio cholerae O1 biotype El Tor serotype Ogawa associated with an outbreak in Kolkata, India
Shyamapada Mandal,Manisha DebMandal,Nishith Kumar Pal
Asian Pacific Journal of Tropical Medicine. 2010; 3(8): 637
[Pubmed] | [DOI]
3 An outbreak of cholera in and around Aurangabad
Duthade, M.M., Bhakre, J.B., Gaikwad, A.A., Khaparkuntikar, M.N., Damle, A.S.
Journal of Communicable Diseases. 2010; 42(2): 165-167
[Pubmed]
4 Circulating phage type of Vibrio cholerae in Mysore
Srirangaraj, S., Venkatesha, D.
Indian Journal of Medical Microbiology. 2009; 27(2): 166-167
[Pubmed]
5 Isolation of Vibrio cholerae El Tor serotype Inaba in 2006 and the most common phage type of V. cholerae in Mumbai
De, A., Mathur, M.
Indian Journal of Community Medicine. 2009; 34(1): 78
[Pubmed]



 

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