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 ORIGINAL ARTICLE
Year : 2006  |  Volume : 24  |  Issue : 3  |  Page : 186-194

DNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic locales


1 Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka
2 Faculty of Dentistry, University of Hong Kong, Hong Kong SAR, China

Correspondence Address:
L P Samaranayake
Faculty of Dentistry, University of Hong Kong, Hong Kong SAR
China
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Source of Support: None, Conflict of Interest: None


PMID: 16912438

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Purpose: To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen. Methods: A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns. Results: Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (SAB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region. Conclusions: This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis .






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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