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CASE REPORTS
Year : 2005  |  Volume : 23  |  Issue : 4  |  Page : 267-269
 

Human babesiosis - A case report


Pranav Pathology Laboratory, Raopura, Baroda : 390 001, Gujarat, India

Correspondence Address:
A Marathe
Pranav Pathology Laboratory, Raopura, Baroda : 390 001, Gujarat
India
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Source of Support: None, Conflict of Interest: None


PMID: 16327127

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 ~ Abstract 

Babesiosis is an emerging, tick-transmitted, zoonotic disease caused by hematotropic parasites of the genus Babesia. Most cases of Babesial infections in humans have been acquired in temperate regions of the United States, Europe, France and England. A few cases of Babesiosis have been described in other parts of the world, including China, Taiwan, Egypt, South Africa, and Mexico.1,2 We report the first case of human Babesiosis, in a normosplenic, previously healthy individual from India.


Keywords: Babesia, Piroplasmosis


How to cite this article:
Marathe A, Tripathi J, Handa V, Date V. Human babesiosis - A case report. Indian J Med Microbiol 2005;23:267-9

How to cite this URL:
Marathe A, Tripathi J, Handa V, Date V. Human babesiosis - A case report. Indian J Med Microbiol [serial online] 2005 [cited 2018 Dec 12];23:267-9. Available from: http://www.ijmm.org/text.asp?2005/23/4/267/17084


 Babesiosis More Details, caused by infection with intraerythrocytic parasites of the genus  Babesia More Details , is one of the most common infections of free-living animals worldwide and is gaining increasing interest as an emerging zoonosis in humans. All Babesial parasites described to date are transmitted by Ixodid ticks to their vertebrate hosts, but the infection can spread by blood transfusion and perinatally. The parasites replicate in the vertebrate hosts' red blood cells and are called piroplasms due to their pear-shaped appearance when within the infected host cells.[1],[2] Human babesiosis is caused by one of several babesial species that have distinct geographic distributions based on the presence of competent hosts.

In North America, babesiosis is caused predominantly by Babesia microti[3] a rodent-borne piroplasm, and also occasionally by a newly recognized species, the so-called WA1 piroplasm.[4] In Europe, babesiosis is considerably rare but more lethal; it is caused by the bovine pathogen Babesia divergens . The spectrum of disease is broad, ranging from an apparently silent infection to a fulminant, malaria-like disease resulting occasionally in death.

Without passing through pre-erythrocytic stage the Babesia parasites enter the erythrocytes and multiply resulting in rupture of the RBCs followed by infection of other RBCs.[5]


 ~ Case Report Top


A fifty-one-year old male patient presented in a private clinic in Baroda (Gujarat) with the complaints of fever for one day, anorexia and vomiting. He was advised amoxycillin 500 mg twice daily and paracetamol.

The patient was a resident of Gwalior (Madhya Pradesh) and had come to Baroda only for a short period of time. At Gwalior he was working in a nursing home. He was non-diabetic, did not use tobacco or drugs. No other family member had fever or any other illness. He had no history of splenectomy or blood transfusion.

On the following day the patient got very high grade fever and had taken 650 mg of paracetamol. He was brought to the hospital where after examination he was admitted with the complaints of high grade fever, anorexia, vomiting, profuse sweating, chills, mild headache and arthralgia.

On examination, the patient had fever of 1020 F, pulse rate 97/minute, blood pressure of 110/70 mm. of Hg. The liver and spleen were palpable. The patient had scleral icterus. He was passing dark coloured urine. The investigations done are shown in the table.

The peripheral blood smear was stained with Leishman's stain. The ring forms visible within erythrocytes varied greatly and were confused with Plasmodium species especially with falciparum [Figure - 1]a. The patient was reported of having P. falciparum and was treated with antimalarial drugs. The density of parasite was about 5%. The typical pairs and tetrads of pear-shaped trophozoites were seen at many places in the erythrocytes [Figure - 1]b, [Figure - 1]c. Also were seen schizont forms devoid of haemozoin pigment [Figure - 1]d, the extra cellular merozoites [Figure - 1]e and intra erythrocytic multiple merozoites [Figure - 1]f. After complete and careful examination of smears the parasites were identified by their morphology as Babesia species.

To rule out co-infection with Plasmodium species, tests were performed to detect P. falciparum antigen (HRP II) and other Plasmodium specific antigen, which turned out negative. The serum was also negative for HIV (I & II) antibodies. The patient was put on clindamycin 250 mg four times daily and quinine 300 mg. per day for seven days and was also given folinic acid 15 mg diluted in 100 mL saline for the low WBC counts and thrombocytopenia. The patient became afebrile after two days. The repeat peripheral smears after seven days did not show any blood parasites, the total WBC and platelet counts became normal. The patient was discharged.


 ~ Discussion Top


Human babesiosis is caused by one of several babesial species that have distinct geographic distribution based on the presence of competent hosts. The spectrum of disease is

broad, ranging from an apparently silent infection to a fulminant, malaria-like disease resulting occasionally in

death. Various determinants are involved in the severity of

disease manifestation; among those identified are age, immunocompetence, and coinfection with other pathogenic agents.[6]

The diagnosis of babesiosis should begin with a descriptive history, which might include appropriate clinical manifestations, history of travel to an area where it is endemic, tick bite or exposure to a tick-infested area, recent blood transfusion and splenectomy.[6]

Babesia microti is an intracellular parasite within the erythrocytes; therefore, diagnosis of human babesiosis has relied heavily upon the determination of the presence of the erythrocytic stage of the organism. The tetrad forms (Maltese-cross) of the parasite are believed to be the primary diagnostic character for the disease. However, the predominant forms in most of the blood smears closely resemble rings of Plasmodium spp., with small to large cytoplasmic vacuoles. Therefore, it is difficult to differentiate Babesia microti from Plasmodium spp., especially Plasmodium falciparum . It is recommended that diagnosis of clinical case of human babesiosis be made by a combination of criteria including the presence of intense parasitaemia (1-50%), erythrocytes infected by multiple basket-shaped parasites, and the presence of extracellular merozoites.[7] In most instances, however, an accurate patient history, clinical presentation, and observation of characteristic morphologic features are sufficient to establish the appropriate diagnosis; otherwise molecular techniques may be used.[6]

The serologic tests may be performed if the parasitaemia is low but does not replace smear examination because of cross reaction of antibodies with other blood parasites.[8] The expensive technique like PCR is performed only by the reference laboratories for the diagnosis of the species of Babesia.

Our patient did not give history of tick bite or recent visit to any of the endemic areas which suggests an indigenous source of infection. During the smear examination at many places the paired pear-shaped merozoites, tetrads, multiple merozoites, absence of hemozoin pigment in the schizont forms and extra cellular merozoites were observed. The absence of HRP II, jaundice, low total WBC count and thrombocytopaenia and unremarkable history of patient suggested an infection with a different intraerythrocytic blood parasite. Thorough review of the literature on babesiosis and the comparative smear examination led us to the diagnosis of this rare and probably the first case of human babesiosis in India.

Malaria is endemic in many parts of India and the intraerythrocytic parasites are frequently reported as plasmodia. Careful examination of peripheral blood smears can only differentiate the Babesia from malarial parasites. Thorough peripheral smear examination and serological surveys may be necessary to know the actual prevalence of human babesiosis in India.





 
 ~ References Top

1.Kakoma I, Mehlhorn H. Babesia of domestic animals, In : Kreier JP, editors. Parasitic protozoa . 2nd ed. (Academic Press: San Diego) 1993. p. 141-216.  Back to cited text no. 1    
2.Telford SR. III, Gorenflot A, Brasseur P, Spielman A . Babesial infections in humans and wildlife . 1993;1-47.   Back to cited text no. 2    
3.Dammin GJ, Spielman A, Benach JL, Piesman J . The rising incidence of clinical Babesia microti infection. Hum Pathol 1981; 12 :398-400.  Back to cited text no. 3    
4.Persing DH, Herwaldt BL, Glaser C, Lane RS, Thomford JW, Mathiesen D, et al . Infection with a babesia-like organism in northern California. N Engl J Med 1995; 332 :298-303.   Back to cited text no. 4    
5.Gorenflot AK, Moubri E, Carcy PB, Schetters TP. Human babesiosis. Ann Trop Med Parasitol 1998; 92 :489-501.   Back to cited text no. 5    
6.Homer MJ, Aguilar-Delfin I, Telford SR 3rd, Krause PJ, Persing DH. Babesiosis. Clin Microbio Rev 2000; 13: 451-69.  Back to cited text no. 6    
7.McKay K, Sikand T, Lepore L, Christianson C, Persing T, Spielman R. Deer-Associated Infection Study Group. Clin Infect Dis 2002: 34 ;1184-91.  Back to cited text no. 7    
8.Parry MF, Fox M, Burka SA, Richar WJ. Babesia microti infection in man. JAMA 1977; 238 :1282-3.  Back to cited text no. 8    


Figures

[Figure - 1]

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