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 BRIEF COMMUNICATIONS
Year : 2005  |  Volume : 23  |  Issue : 4  |  Page : 256-258

Sterilisation of extracted human teeth for educational use


1 Department of Community Dentistry, Kasturba Medical College, Mangalore-575 001, Karnataka, India
2 Department of Microbiology, Kasturba Medical College, Mangalore-575 001, Karnataka, India

Correspondence Address:
G K Bhat
Department of Microbiology, Kasturba Medical College, Mangalore-575 001, Karnataka
India
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Source of Support: None, Conflict of Interest: None


PMID: 16327123

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Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100C for 20 minutes, group 5 teeth were autoclaved at 121C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04