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CORRESPONDENCE
Year : 2005  |  Volume : 23  |  Issue : 3  |  Page : 208
 

Antibiotic Susceptibility Pattern and Plasmid Profile of Multidrug Resistant Salmonella typhi


1 Dept of Microbiology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India
2 Dept of Genetics, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India

Date of Submission20-Sep-2004
Date of Acceptance08-Feb-2005

Correspondence Address:
K R Reddy
Dept of Microbiology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.16601

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How to cite this article:
Reddy K R, Rajesh P K, M, Sekar U. Antibiotic Susceptibility Pattern and Plasmid Profile of Multidrug Resistant Salmonella typhi. Indian J Med Microbiol 2005;23:208

How to cite this URL:
Reddy K R, Rajesh P K, M, Sekar U. Antibiotic Susceptibility Pattern and Plasmid Profile of Multidrug Resistant Salmonella typhi. Indian J Med Microbiol [serial online] 2005 [cited 2019 Sep 17];23:208. Available from: http://www.ijmm.org/text.asp?2005/23/3/208/16601


Dear Editor,

Enteric fever continues to be a major health problem in India and plasmid mediated resistance in  Salmonella More Details typhi is known since 1972.[1] Sixty three strains of S. typhi were isolated from blood and bone marrow cultures at the microbiology laboratory of Sri Ramachandra Medical College and Research Institute, Chennai, during the period, March 2002 to September 2002. Susceptibility to ampicillin, co-trimoxazole, chloramphenicol and Tetracycline (ACCOT) was determined by disc diffusion method. Minimal inhibitory concentration (MIC) to all the above drugs were determined by agar dilution method according to NCCLS standards using ATCC E.coli 25922 as control.[2] ACCOT resistance was seen in 35 strains both by disc diffusion and MIC determination. The MIC values of the resistant strains were -ampicillin 2048 g/mL, chloramphenicol 512 g/mL, trimethoprim 160 g/mL, sulfamethoxazole 128 g/mL and tetracycline 1024 g/mL. No resistance to ciprofloxacin, cefotaxime and ceftriaxone were observed by disc diffusion. However, the results have to be evaluated further by performing the MIC for these drugs. also. These strains were subjected to alkaline lysis to isolate high molecular weight plasmid DNA and the plasmid was further analysed by restriction analysis. All the resistant strains carried a plasmid of 23 KB, and showed same restriction pattern with EcoR-1 enzyme and Hind III enzyme. No plasmids were identified in the susceptible strains. ACCOT resistance is still common in S. typhi though declining with increased use of fluoroquinolones and cephalosporins for treatment.[3] A continuous epidemiological surveillance is necessary and plasmid analysis is mandatory for studying the mechanism of drug resistance.



 
 ~ References Top

1.Panikar CKG, Vimala KM, Transferable chloramphenicol resistance in S. typhi. Nature 1972; 239 :199-200.  Back to cited text no. 1    
2.National community for clinical laboratory standards. Performance standards for anti-microbial susceptibility testing 12th information supplement. M100 S 12, Vol. O No. O Wayne PA NCCLS 2000.  Back to cited text no. 2    
3.Jesudasan MV. Reemergence of chloramphenicol sensitive strains. Epidemiol Infect 1996; 116 :225-7.   Back to cited text no. 3    




 

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2004 - Indian Journal of Medical Microbiology
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