Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 951 Official Publication of Indian Association of Medical Microbiologists 
  Search
 
 ~ Next article
 ~ Previous article 
 ~ Table of Contents
  
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~  Article in PDF (43 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 
 ~  Abstract
 ~  Case Report
 ~  Discussion
 ~  References
 ~  Article Figures

 Article Access Statistics
    Viewed10916    
    Printed233    
    Emailed11    
    PDF Downloaded321    
    Comments [Add]    
    Cited by others 21    

Recommend this journal

 


 
CASE REPORT
Year : 2005  |  Volume : 23  |  Issue : 3  |  Page : 195-197
 

Invasive aspergillosis involving multiple paranasal sinuses - A case report


1 Department of Microbiology, Indira Gandhi Medical College, Shimla, Himachal Pradesh - 171 001, India
2 Department of Otorhinolaryngology, Indira Gandhi Medical College, Shimla, Himachal Pradesh - 171 001, India

Date of Submission30-Sep-2004
Date of Acceptance17-Dec-2004

Correspondence Address:
S Agarwal
Department of Microbiology, Indira Gandhi Medical College, Shimla, Himachal Pradesh - 171 001
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.16595

Rights and Permissions

 ~ Abstract 

A case of invasive multiple paranasal sinus aspergillosis with bony involvement is reported. A young immunocompetent lady presented with bilateral nasal obstruction due to polyps. Radiologically and histopathologically a fungal cause was kept a possibility, and the diagnosis of Aspegillus fumigatus was established by demonstration of acute angle branching septate hyphae on direct wet mount and repeated isolation in culture. Patient responded favourably to surgical excision of polyps and oral itraconazole post operatively.


Keywords: Aspergillus, aspergillosis, paranasal sinuses


How to cite this article:
Agarwal S, Kanga A, Sharma V, Sharma D R, Sharma M L. Invasive aspergillosis involving multiple paranasal sinuses - A case report. Indian J Med Microbiol 2005;23:195-7

How to cite this URL:
Agarwal S, Kanga A, Sharma V, Sharma D R, Sharma M L. Invasive aspergillosis involving multiple paranasal sinuses - A case report. Indian J Med Microbiol [serial online] 2005 [cited 2019 Jul 21];23:195-7. Available from: http://www.ijmm.org/text.asp?2005/23/3/195/16595


Aspergillus spp. rank closely behind Candida spp. in causing invasive fungal infections in humans. Primary lesions can be localized in the eyes, paranasal sinuses, external ears and larynx in apparently healthy individuals.[1] There are more than 185 species of Aspegillus and over 95% of all infections are caused by A. fumigatus, A. flavus and A. niger. A. fumigatus alone accounts for the large majority of cases of both invasive and noninvasive aspergillosis.[2] Paranasal sinus aspegillosis includes a spectrum of disease classically described to be four types: allergic, noninvasive, invasive and fulminant.[3] Exposure to Aspergillus spp. is universal but clinical syndrome and pathologic spectrum depends on underlying lung architecture, host's immune response and degree of inoculum. The nose and paranasal sinuses have local factors which promote fungal infection including nasal polyps, recurrent bacterial infections, neutropenia and chronic rhinitis with stagnation of nasal secretions. Other underlying factors include prolonged antibiotic therapy, immunosuppressive drugs and corticosteroids.[2] Acute Aspergillus sinusitis is a life threatening condition encountered in immunocompromised patients. Though decreased immune response has been described as of paramount importance in aspergillosis, review of literature shows that cases of invasive and semi-invasive aspergillosis in non-neutropenic patients have occurred.[4]. Any type of paranasal aspergillosis may progress to or be associated with more aggressive disease illustrating the importance of early recognition of this increasingly encountered disease.


 ~ Case Report Top


A 28 year old female presented with two month history of nasal obstruction and bilateral swelling medial to medial canthus of the eyes in August 2004 in the department of Otorhinolaryngology. She had complaints of repeated episodes of nasal obstruction for past many years with regression of symptoms temporarily. Onset was insidious starting on right side and gradual progression to bilateral total nasal blockside. Patient subsequently developed breathing through mouth and bilateral watering of eyes more on the right side. She complained of scanty seropurulent, odourless nasal discharge. She denied epistaxis, excessive sneezing, headache, paranasal discharge or trauma to nose. History of recurrent upper respiratory infections including fever, cough, sputum production, ear discharge, earache, tinnitus, vertigo, ataxia and asymmertry of face or facial pain were negative. She had no history of tuberculosis, diabetes mellitus asthma, use of corticosteroids, other immunosuppressants or prolonged antibiotic therapy.

On general physical examination, she was afebrile with a blood pressure of 124/80 mm of Hg and pulse rate of 95 per minute. All other vital parameters were normal. Anterior rhinoscopy of revealed bilateral pale white nasal polyps. Polyps were non- tender and probe could be moved all around the growth. Nasal mucosa was normal with no bleed on touch. Bilateral nares blockage was assessed by patency test. There was no abnormal finding on posterior rhinoscopy. Irregular, tender swellings were palpable on both sides medial to medial canthus of eyes with upward extension on the upper eyelids. Ethmoidal sinus was also tender. Clinical examination of ears and throat was normal.

Laboratory values were notable for haemoglobin of 12 gm%. The white blood cell count was 10.8 x 106sub/L with 69% segmented neutrophils, 25% lymphocytes, 4% monocytes, 2% eosinphils and no basophils. Random blood glucose of 107 mg% and fasting level of 59 mg% were recorded. An HIV test was done as there was no clinical suspicion that the patient was immunocompromised.

Plain, contrast and high resolution CT scan for nasal sinus showed soft tissue density masses in maxillary, ethmoid and sphenoid sinuses. There was bony destruction of medial wall of right maxillary antrum with high density probably calcareous deposition. Attenuation of nasal septum, ethmoid trabeculae and uncinate process were observed. Identification of infundibulum, laminae papyraceae and right orbit were also present.

Provisional diagnosis of sinonasal polyposis possibly due to fungal cause with infiltration into nasal cavity and right orbit was made. Patient was started on roxithromycin 300mg per day and advised "functional endoscopic sinus surgery". During surgery, multiple nasal polyps coming out of right maxillary sinus opening and middle meatus on the left side were excised. The lesion yielded a characteristic caseous cheesy material coming from the maxillary sinus which was subsequently curetted. She was started on gatifloxacin. Excised tissue was received for wet mount preparation and culture in sterile saline. Microscopic examination of the wet mount prepartion revealed necrotic tissue and fungal hyphae measuring 3mm to 6mm in diameter. Hyphae were septate, hyaline and showing branching at acute angles. [Figure - 1]a-b

Culture on Sabouraud dextrose agar (SDA) with chloramphenicol were incubated at 37 oC and 25 oC. On day three of incubation, few white fluffy colonies with yellow to green centre were seen on the obverse with a yellow pigment on the reverse. Colonies turned grayish green by day ten. Lactophenol cotton blue wet mount prepared from the fungal growth demonstrated septate hyphae from which nonseptate short smooth condidiophores terminating in vesicle. The features were consistent with the diagnosis of Aspergillus fumigatus .[5]

Histopathological examination of the fibrinopurulent mass revealed abundant septate fungal hyphae. Three days hence patient was kept for endoscopic examination and biopsy was taken. Subsequent culture grew A. fumigatus on day eighth of incubation. Histopathological examination showed necrosis, acute inflammatory cell infiltration and inflammatory cell infiltration and inflammatory granulation tissue. Fungal hyphae were not seen on routine haematoxylin and eosin staining and Grocott silver staining could not be performed. Patient was started on oral itraconazole 400 mg/day. She was reviewed for endoscopic examination after one month. Assessment revealed few bilateral small nasal polyps which were removed from ethmoid gallery and sent for histopathological examintion. Antifungal therapy was continued and patient showed dramatic improvement. [Figure - 2], [Figure - 3]


 ~ Discussion Top


Since first reported by Katzenstein and her colleagues in 1983 there have been few further reports of allergic Aspergillus sinusitis (AAS). The condition is still poorly recognized by both clinicians and pathologists with diagnosis and characterization continuing to be controversial. AAS is considered essentially a saprophytic condition with a benign course. Interestingly, however, Dunlop and Bilson described a case that resulted in visual failure due to orbital involvement.[3] Thus a spectrum of disease severity beginning with benign AAS can be demonstrated. Despite such a capacity for destruction, tissue invasion by Aspergillus has not yet been described in this condition. Sinusitis due to Aspergillus may present in two different forms. The acute life threatening form is seen in immunocompromised patients. Paranasal sinus granuloma formation is a slowly progressive condition as seen in the present case. The typical patient of allergic fungal sinusitis is an immunocompetent, atopic, young adult with a long standing history of allergic rhinitis, nasal congestion, headache, polyposis, asthma and/or recurrent sinusitis. The mean age group of 34.6 +/- 14 years has been reported by Chakrabarti et al [6] from Chandigarh. There is a male preponderance with male to female ration ranging from 1.5:1 to 3:1.[2],[6] On examination, polyps may be evident with associated mucopurulent discharge, enlarged turbinates, hyperplastic nasal mucosa and occasional facial pain.

CT scan has a role in demonstrating extent of lesion and bone destruction. Usually, there is involvement of posterior sphenoid, ethmoid septa, laminarPapyracea and medial antral wall. Bony involvement is reported in 30 to 50% cases.[2] at surgery apart from the polyps, the most striking feature is the thick green- brown to yellow inspissated necrotic or gelatinous mucus often in a cheese like mass.[2],[7] The pres ent case showed the characteristic pattern of bony involvement and cheesy mucus in nasal cavity. Our findings also corroborate with the study of Chakrabarti et al[6] who have reported 95% of patients of allergic fungal rhinosinusitis presenting with nasal polyposis, bilateral involvement in 86%, fungal smear positivity of 56% and presence of hyphae in only 30% on histopathological evaluation. Generally, only the maxillary sinus is involved by Aspergillus. When the disease becomes invasive it spreads from maxillary to ethmoid, sphenoid and rarely frontal sinuses in order of frequency. Extension to orbit and nasal cavity have also been documented. In a review of 103 cases, 67 were found to have solitary sinus involvement with 46 cases affecting the maxillary sinus alone.[2]

The usual fungus isolated is Aspergillus; other cause being phaeoid fungi like Bipolaris, Exserohilum, Curvularia and Alternaria .[2] Demonstration of Aspergillus spp. by both culture and microscopic examination of tissue provides the most firm diagnosis.[1] The appearance of fungal hyphae on histopathological examination, though highly suggestive, is not always seen on routine haematoxylin and eosin stains.[7] The positive results of wet mounts and repeated isolation on culture confirms the dianosis of A.fumigatus beyond doubt in this case. Reports of histopathology are supportive evidence. The particular Aspergillus spp. causing infection may depend on geographical location. Case reports of paranasal sinus mycoses from India and Sudan have reported A.flavus as the cause of majority of cases. [6],[7],[8] The isolate in our case is A.fumigatus which is rarer. The differential diagnosis of allergic bronchopulmonary aspergillosis could be set aside by the absence of pyrexia, recurrent wheezing bronchitis, sputum production and blood eosinophilia. Our patient was clinically reviewed by endoscopic visualization and continued on oral itraconazole 400mg/day for ten days after which the patient became symptom free. In conclusion, we emphasize that awareness regarding the possibility of invasive paranasal sinus aspergillosis is required when examining patients with nasal polyposis in healthy immunocompetent young adults.



 
 ~ References Top

1.Mitchell RB, Tolley N, Croft CB, Gallimore A. Aspergillosis of the larynx. J Laryngol Otol 1994; 108 :883-5.  Back to cited text no. 1    
2.Richardson MD. Aspergillus and Penicllium species Part vii: Monomorphic septate filamentous systemic pathogenic fungi. In : Topley and Wilson's Microbiology and Microbial Infections 9th edn. Ajello L, Hay RJ Editors. (Oxford University Press, New York) 1998. p. 281-312.   Back to cited text no. 2    
3.Jones JR. Paranasal aspergillosis - a spectrum of disease. J Laryngol Otol 1993; 108 :773-4.  Back to cited text no. 3    
4.Nakahira M, Saito H, Miyagi T. Left Vocal Cord Paralysis as a Primary Manifestation of Invasive Pulmonary aspergillosis in a Nonimmunocompromised host. Arch Otolaryngol Head Neck Surg 199; 125 :691-3.  Back to cited text no. 4    
5.Kwon Chung KJ, Bennett JE. Subcutaneous and Deep Mycoses Part IV: Aspergillosis. In : Medical Mycology Cann C, Hunsberger S Editors.Philadelphia; 1992. p. 201-47.  Back to cited text no. 5    
6.Panda NK, Chakrabarti A, Das A, Bapuraj RJ, Saravanan K. To study the prevalence of allergic fungal rhinosinusitis amonh the patients with chronic sinusitis. In: 5th National Conference, Society for Indian Human and Animal Mycologists, Abstract Book 2004. p. 51.  Back to cited text no. 6    
7.Jonathan D, Lund V, Milroy C. Allergic Aspergillus Sinusitis - an overlooked diagnosis? J Laryngol Otol 1989; 103 :1181-3.  Back to cited text no. 7  [PUBMED]  
8.Joseph, David K, Ghosh A., Sharma M, Chakrabarti A. NDA Based Typing Methods for Aspergillus flavus. In : Mycotic Infections in The 21st Century 1st edn. Venogopal, PV, Venogopal TV Editors. (Udayam Offset, Chennai) 2002. p. 240-51.  Back to cited text no. 8    


Figures

[Figure - 1], [Figure - 2], [Figure - 3]

This article has been cited by
1 Potential Role of18F-FDG PET/CT in Patients With Fungal Infections
Punit Sharma,Anirban Mukherjee,Sellam Karunanithi,Chandrasekhar Bal,Rakesh Kumar
American Journal of Roentgenology. 2014; 203(1): 180
[Pubmed] | [DOI]
2 Acute Chiasmal Abscess Resulting from Perineural Extension of Invasive Sino-Orbital Aspergillosis in an Immunocompetent Patient
Ilya Leyngold,Alessandro Olivi,Masaru Ishii,Ari Blitz,Peter Burger,Prem S. Subramanian,Gary Gallia
World Neurosurgery. 2013;
[Pubmed] | [DOI]
3 Invasive maxillary aspergillosis masquerading as malignancy in two cases: Utility of cytology as a rapid diagnostic tool
Sharma, D. and Khurana, N. and Rao, S. and Mahajan, N. and Jain, S.
Journal of Cytology. 2012; 29(3): 194-196
[Pubmed]
4 In vitro effect of honey on Aspergillus species isolated from different clinical infections
Pradeep, P.R. and Dhananjay, D.S.
Journal of Pure and Applied Microbiology. 2012; 6(3): 1363-1367
[Pubmed]
5 Chronic fungal rhinosinusitis due to Aspergillus versicolor
Ghadage, D.P. and Mali, R.J. and Wankhade, A.B. and Bhore, A.V.
Indian Journal of Otology. 2012; 18(2): 103-105
[Pubmed]
6 Aspergillus infection of paranasal sinuses
Khan, M.A. and Rasheed, A. and Awan, M.R. and Hameed, A.
Journal of Taibah University Medical Sciences. 2010; 5(2): 60-65
[Pubmed]
7 Radiological aspects of the ethmoidal polyps: A tertiary care experience
Rasheed, A. and Awan, M.R. and Ahmad, S.A. and Khan, M.A. and Hameed, A.
Pakistan Journal of Medical and Health Sciences. 2010; 4(4)
[Pubmed]
8 A unique cytological approach in diagnosing a case of invasive aspergillosis masquerading as retro-orbital neoplasm
Khanna, M. and Mannan, R. and Khanna, A. and Mannan, R. and Kaur, J.
Journal of Clinical and Diagnostic Research. 2010; 4(2): 2311-2315
[Pubmed]
9 Vertebral osteomyelitis with a rare etiology diagnosed by fine-needle aspiration cytology
Nandeesh, B.N., Kini, U., Alexander, B.
Diagnostic Cytopathology. 2010; 38(5): 360-363
[Pubmed]
10 Fine-needle aspiration biopsy as an initial diagnostic modality in a clinically unsuspected case of invasive maxillary fungal sinusitis: A case report
Singh, N., Siddaraju, N., Kumar, S., Muniraj, F., Bakshi, S., Gopalakrishnan, S.
Diagnostic Cytopathology. 2010; 38(4): 290-293
[Pubmed]
11 Aspergillus Infection of Paranasal Sinuses
Maroof Aziz Khan,Abid Rasheed,Muhammad Rashid Awan,Azhar Hameed
Journal of Taibah University Medical Sciences. 2010; 5(2): 60
[Pubmed] | [DOI]
12 Vertebral osteomyelitis with a rare etiology diagnosed by fine-needle aspiration cytology
BN Nandeesh,Usha Kini,Betty Alexander
Diagnostic Cytopathology. 2009; : NA
[Pubmed] | [DOI]
13 Fine-needle aspiration biopsy as an initial diagnostic modality in a clinically unsuspected case of invasive maxillary fungal sinusitis: A case report
Neha Singh,Neelaiah Siddaraju,Surendra Kumar,Femela Muniraj,Satvinder Bakshi,Suryanarayanan Gopalakrishnan
Diagnostic Cytopathology. 2009; : NA
[Pubmed] | [DOI]
14 Invasive Aspergillosis of the Paranasal Sinuses Invading Skull Base : Successful Treatment with Voriconazole
Chai Ryoung Eun, Jung Yeon Heo, Ji Yun Noh, Young Kyung Youn, Hyun Joo Lee, Bong Kyung Shin, Jacob Lee, Joon Young Song, Hee Jin Cheong, Woo Joo Kim
Infection and Chemotherapy. 2008; 40(2): 110
[VIEW] | [DOI]
15 Allergic fungal sinusitis, fungus ball and invasive sinonasal mycosis - Three fungal-related diseases | [Allergische Pilzsinusitis, Fungusball und invasive Mykose der Nasennebenhöhlen - Drei distinkte, pilzinduzierte Krankheitsbilder]
Driemel, O., Wagner, C., Hurraß, S., Müller-Richter, U., Kühnel, T., Reichert, T.E., Kosmehl, H.
Mund - Kiefer - und Gesichtschirurgie. 2007; 11(3): 153-159
[Pubmed]
16 Isolation of Aspergillus nidulans from a case of fungal rhinosinusitis: A case report
Shah, P.D., Deokule, J.S.
Indian Journal of Pathology and Microbiology. 2007; 50(3): 677-678
[Pubmed]
17 Chronic invasive sinus aspergillosis in an immunocompetent patient: A case report
Mylona, S., Tzavara, V., Ntai, S., Pomoni, M., Thanos, L.
Dentomaxillofacial Radiology. 2007; 36(2): 102-104
[Pubmed]
18 Allergic fungal sinusitis, fungus ball and invasive sinonasal mycosis - Three fungal-related diseases [Allergische Pilzsinusitis, Fungusball und invasive Mykose der Nasennebenhöhlen - Drei distinkte, pilzinduzierte Krankheitsbilder]
Driemel, O. and Wagner, C. and Hurraß, S. and Müller-Richter, U. and Kühnel, T. and Reichert, T.E. and Kosmehl, H.
Mund - Kiefer - und Gesichtschirurgie. 2007; 11(3): 153-159
[Pubmed]
19 Pulmonary fungal ball in non-immunocompromised patient: A case report
Jain, R., Agarwal, D., Lahiri, T.K., Tilak, V., Gulati, A.K.
Indian Journal of Pathology and Microbiology. 2007; 50(4): 930-933
[Pubmed]
20 Chronic invasive sinus aspergillosis in an immunocompetent patient: a case report
S Mylona,V Tzavara,S Ntai,M Pomoni,L Thanos
Dentomaxillofacial Radiology. 2007; 36(2): 102
[Pubmed] | [DOI]
21 Allergische Pilzsinusitis, Fungusball und invasive Mykose der Nasennebenhöhlen – drei distinkte, pilzinduzierte Krankheitsbilder
Oliver Driemel,Christina Wagner,Susann Hurraß,Urs Müller-Richter,Thomas Kühnel,Torsten Eugen Reichert,Hartwig Kosmehl
Mund-, Kiefer- und Gesichtschirurgie. 2007; 11(3): 153
[Pubmed] | [DOI]



 

Top
Print this article  Email this article
Previous article Next article

    

© 2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04