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CASE REPORT
Year : 2005  |  Volume : 23  |  Issue : 3  |  Page : 192-194
 

Mycobacterium avium intracellularae complex associated extrapulmonary axillary lymphadenitis in a HIV-seropositive infant - A rare case report


1 Department of Pathology, MKCG Medical College, Berhampur 760 004, Ganjam Orissa, India
2 Department of Microbiology, MKCG Medical College, Berhampur 760 004, Ganjam Orissa, India
3 Department of Paediatrics, MKCG Medical College, Berhampur 760 004, Ganjam Orissa, India

Date of Submission10-Sep-2004
Date of Acceptance04-Nov-2004

Correspondence Address:
S Mahapatra
Department of Pathology, MKCG Medical College, Berhampur 760 004, Ganjam Orissa
India
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DOI: 10.4103/0255-0857.16594

PMID: 16100429

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 ~ Abstract 

Opportunistic infections by Mycobacterium avium intracellulare complex in HIV infected patients, though common in adults, are rarely seen in infants. We herewith report an interesting case of an eight month old infant presenting with isolated axillary lymphadenitis, later on diagnosed to be tubercular lymphadenitis by Mycobacterium avium intracellulare and finally proved to be seropositive for HIV infection born to previously undetected HIV seropositive parents.


Keywords: Mycobacterium avium intracellulare, extrapulmonary, axillary lymphadenitis


How to cite this article:
Mahapatra S, Mahapatra A, Tripathy S, Rath G, Dash A K, Mahapatra A. Mycobacterium avium intracellularae complex associated extrapulmonary axillary lymphadenitis in a HIV-seropositive infant - A rare case report. Indian J Med Microbiol 2005;23:192-4

How to cite this URL:
Mahapatra S, Mahapatra A, Tripathy S, Rath G, Dash A K, Mahapatra A. Mycobacterium avium intracellularae complex associated extrapulmonary axillary lymphadenitis in a HIV-seropositive infant - A rare case report. Indian J Med Microbiol [serial online] 2005 [cited 2014 Oct 24];23:192-4. Available from: http://www.ijmm.org/text.asp?2005/23/3/192/16594


The association of HIV infection with tuberculosis was first recognised in Haitians and intravenous dr ug abusers.[1],[2] Tuberculosis is now recognised as one of the most common opportunistic infections seen in HIV seropositive patients, mostly presenting in the form of pulmonary, extrapulmonary and disseminated opportunistic infections. The most frequent form of extrapulmonary tuberculosis in patients with HIV infection are lymphadenitis and milliary disease.[3],[4] The diagnosis of extrapulmonary tuberculosis is usually made presumptively by aspiration biopsy of the lymph nodes along with demonstration of acid fast bacilli in 67-90% of these cases.[5],[6]

Herewith, we report an unusual case of extrapulmonary axillary tubercular lymphadenitis by Mycobacterium avium-intracellulare complex (MAC) in a previously undetected HIV seropositive infant.


 ~ Case Report Top


An eight month old infant was brought to the paediatric out patient department of MKCG Medical College Hospital with clinical features of fever, weight loss and isolated left axillary lymphadenopathy of four week duration. The infant was earlier treated with antimalarials and antibiotics with no response.

Parents gave the history of vaccination against OPV, DPT and BCG. The child was born at home out of non-consanguineous marriage and was from a low socioeconomic status. On examination, the child was cachectic (5 kgs), irritable, febrile and pale. Systemic examination revealed no significant abnormality. The only consistent finding was isolated left axillary lymphadenopathy. The mass was two centimeters in diameter, non-tender and mobile. All haematological parameters were within normal limits and X-ray chest did not reveal any abnormality. Fine needle aspiration cytology (FNAC) of the left axillary lymphnode showed presence of large number of foamy histiocytes admixed with reactive lymphoid cells on a highly necrotic background and absence of epithelioid cells and Langhan's giant cells [Figure - 1]a raised the suspicion of an atypical mycobacterial infection. On Ziehl Neelsen (Z-N) staining of the cytoaspirate large numbers of acid fast bacilli [Figure - 1]b were found both inside and outside these histiocytes. Both the parents and infant were advised for HIV test and all of them were found to be seropositive for HIV I antibody by ERS (ELISA rapid and simple method).

For further confirmation, the cytoaspirate was subjected to culture on Lowenstein Jensen (LJ) medium. LJ medium revealed smooth, discrete, dull white colonies at the end of third week of incubation [Figure - 2]a. ZN staining of these colonies revealed small deeply stained acid fast coccobacilli [Figure - 2]b. The colonies were provisionally identified as Mycobacterium avium Scientific Name Search  - intracellulare complex (MAC) based on the morphology, slow rate of growth, inability to produce pigment, hydrolysis of Tween-80 and the ability to produce heat stable catalase as per stasndard procedures.[7]

A diagnosis of coinfection of extrapulmonary tuberculosis (by atypical mycobacteria MAC) with HIV was made and CD4 cell count and viral RNA load studies were requested. Treatment was started provisionally with clarithromycin 15 mg/kg/day in divided doses and rifabutin 10mg/kg/day. The patient responded dramatically to therapy resulting in gross decrease in the size of the axillary node at the end of second week and the general condition was also improved simultaneously. The CD4 cell count was 412/mL and viral RNA load (by RNA PCR assay) was 20,000 copies/mL. Subsequently, monotheraphy with zidovudin was added and the patient was further kept on follow up.


 ~ Discussion Top


HIV infection has been a major cause of morbidity and mortality since the first case of AIDS among children was reported in 1982 in the United States. Perinatal transmission of HIV accounts for 90% of paediatric AIDS cases and almost all new HIV infection in children.[8] Considerable advances, especially in the past 5 years, in understanding the pathogenesis, diagnosis, treatment, monitoring and prevention of HIV infection in children has changed the epidemiology of pediatric HIV infection all over the world. Mycobacterium avium complex (MAC) typically occurs later in the course of HIV disease with increasing immune suppression. Lymph node tuberculosis accounts for 2.9% of cases in children below one year of age.[9] Lymph node affection by MAC is still rarer in infants. MAC has a significant effect on survival among children, with only a 50% chance of survival of seven months after diagnosis.

Disseminated infection with MAC occurs almost exclusively in children and adults with advanced HIV disease. However, our case presented with only a solitary axillary lymphadenopathy. Mycobacterium avium complex includes 3 closely related species M. avium, M. intracellulare and M. scrofulaceum . They are intracellular organisms that proliferate within macrophages. Defective cell mediated immunity in children with advanced HIV disease results in uncontrolled bacterial replication within the macrophages. Granuloma formation is unusual and pathologic specimens are likely to reveal macrophages filled with many bacilli as in our case. A high tissue burden is found in lungs, liver, spleen, intestine, bone marrow and lymph nodes. Characteristic histologic finding of acid fast bacilli within macrophages are highly suggestive of M. avium infection and may hasten the initiation of therapy in children with a suspected diagnosis, but cultures are imperative to ascertain for species identification. However, successful cultivation of M. avium depends upon the bacillary burden.

Our patient aged 8 months, reported to the hospital with irregular fever, weight loss and isolated left axillary lymphadenopathy of four months duration. The cytoaspirate of the node demonstrated a large number of macrophages on a highly necrotic background, which on ZN staining revealed the characteristic pattern of histiocytes loaded with acid fast bacilli, raising a strong suspicion of HIV infection in the child, which was further confirmed by ELISA test. Culture of the cytoaspirate confirmed the presence of MAC.

Isolated tubercular axillary lymphadenopathy in HIV infected children without any other systemic involvement is extremely rare. Hence, any child above six months of age presenting with isolated extrapulmonary lymphadenopathy must raise a strong suspicion of occult HIV infection even at the early phase of the disease.



 
 ~ References Top

1.Sunderam G, McDonald RJ, Maniatis T,Oleska J, Kapila R, Reichman LB. Tuberculosis as a manifestation of the acquired immunodeficiency syndrome (AIDS), JAMA 1986; 256 :362-6.  Back to cited text no. 1    
2.Pitchenik AE, Fischl MA. Disseminated tuberculosis and the acquired immunodeficiency syndrome. Ann Intern Med 1983; 97 :112.  Back to cited text no. 2    
3.Taliv VH, Khurana SK, Pandey J, Vermask. Current concepts tuberculosis and HIV infection. Indian J Pathol Microbiol JPM , 1993; 36 :503-11.  Back to cited text no. 3    
4.Deodhar L. Mycobacteremia in AIDS patients- Report of 2 cases. Indian J Med Microbiol , 1999; 17 :196-7.  Back to cited text no. 4    
5.Modilovsky T, Sattler FR, Barnes PF. Mycobacterial disease in patients with human immunodeficiency virus infection. Arch Intern Med 1989; 149 :2201-5.  Back to cited text no. 5    
6.Kramer F, Modilevsky T, Waliany AR, Leedom JM, Barnes PF. Delayed diagnosis of tuberculosis in patients with human immunodeficiency virus infection. Am J Med 1990; 89 :451-6.  Back to cited text no. 6  [PUBMED]  
7.Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn JR WC (Eds.), Mycobacteria. In : Color atlas and text book of diagnostic microbioloty. 4th edn. East Washington Square: JB Lippincot Company; 1979. p. 705-39.  Back to cited text no. 7    
8.Centres for disease control and prevention HIV/AIDS surveillance report. 1999; 11 :1-24.  Back to cited text no. 8    
9.Shahab T, Zoha MS, Malik MA, Malik A, Afzal K. Prevalence of human immunodeficiency virus infection in children with tuberculosis. Indian Pediatr 2004; 41 :595-9.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]


Figures

[Figure - 1], [Figure - 2]

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